Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 245956..246497 Replicon chromosome
Accession NZ_CP102379
Organism Escherichia coli strain BM28 lysU

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NR350_RS01145 Protein ID WP_000615983.1
Coordinates 245956..246234 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NR350_RS01150 Protein ID WP_000729703.1
Coordinates 246237..246497 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR350_RS01130 (243538) 243538..244116 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
NR350_RS01135 (244322) 244322..245089 + 768 WP_000333380.1 class II glutamine amidotransferase -
NR350_RS01140 (245060) 245060..245800 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NR350_RS01145 (245956) 245956..246234 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NR350_RS01150 (246237) 246237..246497 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NR350_RS01155 (246707) 246707..247456 + 750 WP_000056849.1 C40 family peptidase -
NR350_RS01160 (247632) 247632..248129 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NR350_RS01165 (248353) 248353..250065 - 1713 Protein_225 flagellar biosynthesis protein FlhA -
NR350_RS01170 (250037) 250037..250822 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island formA / tet(B) gmhA/lpcA 234023..281313 47290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T253528 WP_000615983.1 NZ_CP102379:c246234-245956 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT253528 WP_000729703.1 NZ_CP102379:c246497-246237 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References