Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3244364..3245091 Replicon chromosome
Accession NZ_CP102378
Organism Escherichia coli strain JB41

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NR351_RS15845 Protein ID WP_000550189.1
Coordinates 3244777..3245091 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NR351_RS15840 Protein ID WP_000560266.1
Coordinates 3244364..3244780 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR351_RS15830 (3239524) 3239524..3241875 + 2352 WP_000695487.1 alpha-glucosidase -
NR351_RS15835 (3242301) 3242301..3244319 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NR351_RS15840 (3244364) 3244364..3244780 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NR351_RS15845 (3244777) 3244777..3245091 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NR351_RS15850 (3245375) 3245375..3246511 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NR351_RS15855 (3246596) 3246596..3247099 + 504 WP_001333820.1 M48 family metallopeptidase -
NR351_RS15860 (3247176) 3247176..3247868 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
NR351_RS15865 (3247947) 3247947..3248933 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T253518 WP_000550189.1 NZ_CP102378:c3245091-3244777 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT253518 WP_000560266.1 NZ_CP102378:c3244780-3244364 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References