Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 637370..638169 Replicon chromosome
Accession NZ_CP102244
Organism Escherichia coli O7:H15 strain ST70

Toxin (Protein)


Gene name yhaV Uniprot ID A0A369FDQ4
Locus tag NQZ80_RS03070 Protein ID WP_000347280.1
Coordinates 637370..637834 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NQZ80_RS03075 Protein ID WP_001307405.1
Coordinates 637834..638169 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQZ80_RS03040 (632371) 632371..632805 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NQZ80_RS03045 (632823) 632823..633701 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NQZ80_RS03050 (633691) 633691..634470 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NQZ80_RS03055 (634481) 634481..634954 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NQZ80_RS03060 (634977) 634977..636257 - 1281 WP_000681960.1 tagatose-bisphosphate aldolase subunit KbaZ -
NQZ80_RS03065 (636506) 636506..637315 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
NQZ80_RS03070 (637370) 637370..637834 - 465 WP_000347280.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NQZ80_RS03075 (637834) 637834..638169 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NQZ80_RS03080 (638318) 638318..639889 - 1572 WP_024216579.1 galactarate dehydratase -
NQZ80_RS03085 (640264) 640264..641598 + 1335 WP_024046198.1 galactarate/glucarate/glycerate transporter GarP -
NQZ80_RS03090 (641614) 641614..642384 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17862.29 Da        Isoelectric Point: 9.6924

>T253354 WP_000347280.1 NZ_CP102244:c637834-637370 [Escherichia coli O7:H15]
MDFPQRVNSWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT253354 WP_001307405.1 NZ_CP102244:c638169-637834 [Escherichia coli O7:H15]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A369FDQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References