Detailed information of TA system TA025326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 848327..848945
• Replicon: chromosome
• Accession: NC_015590
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SINME_RS04175
• Protein ID: WP_010969011.1
• Coordinates: 848649..848945 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SINME_RS04170
• Protein ID: WP_010969010.1
• Coordinates: 848327..848635 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS04145	843435..843800	+	366	WP_013844224.1	hypothetical protein	-
SINME_RS37805	844250..844399	+	150	WP_013844225.1	hypothetical protein	-
SINME_RS04150	844536..845738	-	1203	WP_013844226.1	IS256-like element ISRm3 family transposase	-
SINME_RS04155	845929..847116	-	1188	WP_080564414.1	TlpA family protein disulfide reductase	-
SINME_RS37810	847434..847747	+	314	Protein_825	ATP-dependent DNA ligase	-
SINME_RS04165	848086..848308	-	223	Protein_826	IS481 family transposase	-
SINME_RS04170	848327..848635	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SINME_RS04175	848649..848945	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SINME_RS04180	848993..849298	-	306	WP_003527305.1	hypothetical protein	-
SINME_RS04185	849626..850663	+	1038	WP_003527304.1	porin	-
SINME_RS04190	850773..851839	-	1067	Protein_831	site-specific integrase	-
SINME_RS04195	851880..852929	-	1050	WP_013844229.1	porin	-
SINME_RS04200	853143..853631	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	844536..845738	1202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T25326 WP_010969011.1 NC_015590:c848945-848649 [Sinorhizobium meliloti AK83]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T25326 NC_015590:c848945-848649 [Sinorhizobium meliloti AK83]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT25326 WP_010969010.1 NC_015590:c848635-848327 [Sinorhizobium meliloti AK83]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT25326 NC_015590:c848635-848327 [Sinorhizobium meliloti AK83]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61