Detailed information of TA system TA025318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 1049861..1050371
• Replicon: chromosome
• Accession: NC_015581
• Organism: Thioalkalimicrobium cyclicum ALM1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: F6DCT0
• Locus tag: THICY_RS04595
• Protein ID: WP_013835444.1
• Coordinates: 1049861..1050124 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: F6DCT1
• Locus tag: THICY_RS04600
• Protein ID: WP_013835445.1
• Coordinates: 1050129..1050371 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THICY_RS04570	1045348..1046778	-	1431	WP_013835438.1	DegQ family serine endoprotease	-
THICY_RS04575	1046762..1047232	-	471	WP_013835439.1	SoxR reducing system RseC family protein	-
THICY_RS04580	1047333..1048898	+	1566	WP_013835440.1	L-aspartate oxidase	-
THICY_RS04585	1049219..1049434	-	216	WP_013835441.1	twitching motility protein PilT	-
THICY_RS04590	1049421..1049660	-	240	WP_013835442.1	hypothetical protein	-
THICY_RS04595	1049861..1050124	-	264	WP_013835444.1	Txe/YoeB family addiction module toxin	Toxin
THICY_RS04600	1050129..1050371	-	243	WP_013835445.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
THICY_RS04605	1050727..1050945	-	219	WP_013835447.1	hypothetical protein	-
THICY_RS04610	1050929..1051207	-	279	WP_013835448.1	BrnT family toxin	-
THICY_RS04615	1051604..1051987	-	384	WP_013835449.1	type II toxin-antitoxin system VapC family toxin	-
THICY_RS04620	1051991..1052221	-	231	WP_013835450.1	hypothetical protein	-
THICY_RS04625	1052392..1052676	-	285	WP_013835451.1	GIY-YIG nuclease family protein	-
THICY_RS04630	1053134..1053469	-	336	WP_157862721.1	PIN domain-containing protein	-
THICY_RS04635	1053505..1053735	-	231	WP_013835453.1	hypothetical protein	-
THICY_RS04640	1053845..1054243	-	399	WP_013835454.1	tRNA(fMet)-specific endonuclease VapC	-
THICY_RS04645	1054240..1054467	-	228	WP_157862753.1	antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10110.87 Da
• Isoelectric Point: 10.3178

>T25318 WP_013835444.1 NC_015581:c1050124-1049861 [Thiomicrospira cyclica ALM1]
MSWKLVYTKQAVKDAKKLSASGLKSKAQALLELIQQDPFVTPPRFEKLVGDLAGAYSRRINIQHRIVYQVLEDEKLIKVI
RLWTHYE

Nucleotide

• Length: 264 bp

>T25318 NC_015581:c1050124-1049861 [Thiomicrospira cyclica ALM1]
ATGAGTTGGAAGCTGGTTTATACCAAGCAGGCGGTTAAGGATGCAAAAAAGCTGAGTGCCAGTGGGTTGAAGTCTAAAGC
GCAAGCATTGCTCGAATTAATTCAACAAGATCCTTTCGTTACGCCTCCGCGTTTTGAAAAATTGGTTGGCGATTTAGCAG
GTGCCTATTCAAGACGTATTAATATTCAACATCGCATTGTGTATCAGGTGCTAGAGGACGAAAAACTTATTAAAGTTATT
CGCCTTTGGACGCATTATGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8780.93 Da
• Isoelectric Point: 4.1012

>AT25318 WP_013835445.1 NC_015581:c1050371-1050129 [Thiomicrospira cyclica ALM1]
MTSLNSTAARANFYGLIAQVNESHEPITIIGKKGNAVLISEEDWSAINETLFLLSTPGMRESIVEGLKTPVEECDTELVW

Nucleotide

• Length: 243 bp

>AT25318 NC_015581:c1050371-1050129 [Thiomicrospira cyclica ALM1]
ATGACGTCTTTAAATTCTACGGCGGCACGTGCCAATTTTTATGGTTTGATTGCGCAGGTCAACGAATCACATGAACCCAT
TACAATCATAGGTAAGAAAGGCAATGCGGTGCTCATTTCTGAAGAGGATTGGAGCGCGATTAATGAAACCTTGTTTTTGT
TAAGTACGCCTGGGATGCGTGAATCGATTGTTGAAGGTTTGAAAACGCCTGTTGAAGAGTGTGATACTGAGTTAGTTTGG
TAA

Structures

Toxin

• AlphaFold DB: F6DCT0

Antitoxin

• AlphaFold DB: F6DCT1