Detailed information of TA system TA025310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5886..6466
• Replicon: plasmid Lpl
• Accession: NC_015579
• Organism: Novosphingobium sp. PP1Y

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6IBZ0
• Locus tag: PP1Y_RS00290
• Protein ID: WP_013831273.1
• Coordinates: 6185..6466 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: PP1Y_RS00285
• Protein ID: WP_039286522.1
• Coordinates: 5886..6173 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP1Y_RS00270	900..2999	+	2100	WP_041557974.1	ParB/RepB/Spo0J family partition protein	-
PP1Y_RS00275	3101..4153	+	1053	WP_013831269.1	toprim domain-containing protein	-
PP1Y_RS00280	4471..5394	+	924	WP_013831270.1	DUF2493 domain-containing protein	-
PP1Y_RS00285	5886..6173	-	288	WP_039286522.1	HigA family addiction module antidote protein	Antitoxin
PP1Y_RS00290	6185..6466	-	282	WP_013831273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PP1Y_RS00295	6764..7705	-	942	WP_013831274.1	Abi family protein	-
PP1Y_RS00300	8043..8270	+	228	WP_013831275.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
PP1Y_RS00305	8272..8661	+	390	WP_013831276.1	type II toxin-antitoxin system death-on-curing family toxin	-
PP1Y_RS00310	8778..9515	-	738	WP_013831277.1	CDP-alcohol phosphatidyltransferase family protein	-
PP1Y_RS26085	10381..10545	+	165	WP_013831279.1	hypothetical protein	-
PP1Y_RS00315	10686..11129	+	444	WP_013831280.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..192103	192103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (14-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-85)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10398.79 Da
• Isoelectric Point: 5.6832

>T25310 WP_013831273.1 NC_015579:c6466-6185 [Novosphingobium sp. PP1Y]
MIQSAKGKLVNQVLAGKPGKGFPNDIFRAAVRKLAMLEAAVNLDDLRSPPGNRLEALSDDREGQHSIRINDQWRICFVWA
DNGPEDVEIVDYH

Nucleotide

• Length: 282 bp

>T25310 NC_015579:c6466-6185 [Novosphingobium sp. PP1Y]
ATGATACAGAGCGCCAAAGGAAAATTGGTTAATCAGGTGTTGGCGGGCAAACCGGGCAAGGGTTTCCCTAACGACATCTT
CCGGGCTGCTGTGCGGAAGCTGGCAATGCTTGAGGCGGCGGTGAATCTGGACGACTTGCGGTCGCCTCCGGGCAACAGGC
TGGAAGCCCTGTCCGATGACCGTGAGGGCCAGCATTCGATCAGGATCAACGACCAATGGCGTATCTGTTTCGTCTGGGCC
GACAATGGCCCGGAAGATGTAGAGATCGTGGACTATCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.49 Da
• Isoelectric Point: 6.6405

>AT25310 WP_039286522.1 NC_015579:c6173-5886 [Novosphingobium sp. PP1Y]
MTMLKTPVHPGEILKHEFLEEMGISAGKLAKHIHVPRTRIERLCEERTSVTPDTAKRLSKALGTTPEFWLNLQTHYDLLV
DQAADIEEIKPLLAA

Nucleotide

• Length: 288 bp

>AT25310 NC_015579:c6173-5886 [Novosphingobium sp. PP1Y]
ATGACCATGTTGAAAACCCCGGTCCATCCCGGCGAAATCCTCAAGCATGAGTTCCTTGAGGAGATGGGCATTTCCGCAGG
CAAGCTGGCGAAGCATATCCATGTCCCCCGCACCCGCATCGAGCGGCTATGCGAGGAGAGGACTTCGGTCACGCCGGACA
CGGCCAAGCGTCTGTCCAAGGCGCTTGGCACGACGCCGGAGTTCTGGCTCAACCTCCAGACGCATTATGATCTACTGGTG
GACCAGGCCGCTGACATTGAGGAGATTAAGCCGCTGCTGGCGGCTTAA

Structures

Toxin

• AlphaFold DB: F6IBZ0