Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4215997..4216676 Replicon chromosome
Accession NZ_CP102061
Organism Escherichia coli strain C31

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag NP943_RS20460 Protein ID WP_000057523.1
Coordinates 4215997..4216299 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NP943_RS20465 Protein ID WP_000806442.1
Coordinates 4216335..4216676 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP943_RS20435 (4211373) 4211373..4213025 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
NP943_RS20440 (4213063) 4213063..4213566 - 504 WP_000667000.1 hypothetical protein -
NP943_RS20445 (4213563) 4213563..4214363 - 801 WP_000439798.1 hypothetical protein -
NP943_RS20450 (4214387) 4214387..4214866 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NP943_RS20455 (4215070) 4215070..4215864 - 795 WP_000365147.1 TraB/GumN family protein -
NP943_RS20460 (4215997) 4215997..4216299 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NP943_RS20465 (4216335) 4216335..4216676 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NP943_RS20470 (4216734) 4216734..4219238 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
NP943_RS20475 (4219500) 4219500..4220432 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T253094 WP_000057523.1 NZ_CP102061:4215997-4216299 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT253094 WP_000806442.1 NZ_CP102061:4216335-4216676 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References