Detailed information of TA system TA253080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 827629..828131
• Replicon: chromosome
• Accession: NZ_CP102061
• Organism: Escherichia coli strain C31

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: NP943_RS04295
• Protein ID: WP_000767819.1
• Coordinates: 827629..827883 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: NP943_RS04300
• Protein ID: WP_001259253.1
• Coordinates: 827880..828131 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NP943_RS04270 (823447)	823447..823905	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
NP943_RS04275 (824122)	824122..825480	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
NP943_RS04280 (825470)	825470..825532	-	63	WP_010723108.1	membrane protein YoeI	-
NP943_RS04285 (825747)	825747..826676	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
NP943_RS04290 (826722)	826722..827546	-	825	WP_000754737.1	SDR family oxidoreductase	-
NP943_RS04295 (827629)	827629..827883	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
NP943_RS04300 (827880)	827880..828131	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
NP943_RS04305 (828414)	828414..828464	+	51	WP_001364200.1	his operon leader peptide	-
NP943_RS04310 (828610)	828610..829509	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
NP943_RS04315 (829515)	829515..830819	+	1305	WP_001296211.1	histidinol dehydrogenase	-
NP943_RS04320 (830816)	830816..831886	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
NP943_RS04325 (831886)	831886..832953	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	823447..823905	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T253080 WP_000767819.1 NZ_CP102061:c827883-827629 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T253080 NZ_CP102061:c827883-827629 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT253080 WP_001259253.1 NZ_CP102061:c828131-827880 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT253080 NZ_CP102061:c828131-827880 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3