253008

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 43221..43867 Replicon plasmid pEcFELIX377.1
Accession NZ_CP101926
Organism Escherichia coli strain Seattle 1946

Toxin (Protein)


Gene name higB Uniprot ID A0A7R6ZH56
Locus tag NP429_RS25010 Protein ID WP_000269913.1
Coordinates 43520..43867 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag NP429_RS25005 Protein ID WP_001259436.1
Coordinates 43221..43520 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP429_RS24975 (NP429_24985) 39055..39627 - 573 WP_000137931.1 helix-turn-helix transcriptional regulator -
NP429_RS24980 (NP429_24990) 40201..40392 + 192 WP_000183352.1 hypothetical protein -
NP429_RS24985 (NP429_24995) 40389..41531 + 1143 WP_000854799.1 ORF6N domain-containing protein -
NP429_RS24990 (NP429_25000) 41542..41730 - 189 WP_000986262.1 hypothetical protein -
NP429_RS24995 (NP429_25005) 42341..42613 - 273 WP_000148349.1 helix-turn-helix domain-containing protein -
NP429_RS25000 (NP429_25010) 42677..43051 - 375 WP_244426537.1 hypothetical protein -
NP429_RS25005 (NP429_25015) 43221..43520 - 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
NP429_RS25010 (NP429_25020) 43520..43867 - 348 WP_000269913.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NP429_RS25015 (NP429_25025) 44112..44375 - 264 WP_000424604.1 hypothetical protein -
NP429_RS25020 (NP429_25030) 44399..44686 - 288 WP_000356589.1 hypothetical protein -
NP429_RS25025 (NP429_25035) 45330..46310 - 981 WP_228465520.1 plasmid replication initiator RepA -
NP429_RS25030 (NP429_25040) 46492..47046 - 555 WP_001199265.1 recombinase family protein -
NP429_RS25035 (NP429_25045) 47212..48138 + 927 Protein_66 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48488 48488


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13486.53 Da        Isoelectric Point: 9.8723

>T253008 WP_000269913.1 NZ_CP101926:c43867-43520 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT253008 WP_001259436.1 NZ_CP101926:c43520-43221 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7R6ZH56

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References