Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2595100..2595662 Replicon chromosome
Accession NZ_CP101925
Organism Escherichia coli strain Seattle 1946

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag NP429_RS12345 Protein ID WP_000605675.1
Coordinates 2595100..2595378 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag NP429_RS12350 Protein ID WP_000781370.1
Coordinates 2595378..2595662 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP429_RS12320 (2590686) 2590686..2591117 - 432 WP_000152307.1 peroxiredoxin OsmC -
NP429_RS12325 (2591462) 2591462..2591677 + 216 WP_000495766.1 biofilm-dependent modulation protein -
NP429_RS12330 (2591779) 2591779..2591916 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
NP429_RS12335 (2592073) 2592073..2593770 + 1698 WP_000433460.1 malate dehydrogenase -
NP429_RS12340 (2593904) 2593904..2594914 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
NP429_RS12345 (2595100) 2595100..2595378 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NP429_RS12350 (2595378) 2595378..2595662 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
NP429_RS12355 (2595891) 2595891..2596544 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
NP429_RS12360 (2596537) 2596537..2597421 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
NP429_RS12365 (2597434) 2597434..2600481 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T252997 WP_000605675.1 NZ_CP101925:2595100-2595378 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT252997 WP_000781370.1 NZ_CP101925:2595378-2595662 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References