Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 621470..622269 Replicon chromosome
Accession NZ_CP101925
Organism Escherichia coli strain Seattle 1946

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag NP429_RS03050 Protein ID WP_000347252.1
Coordinates 621470..621934 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag NP429_RS03055 Protein ID WP_001296435.1
Coordinates 621934..622269 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP429_RS03020 (616471) 616471..616905 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NP429_RS03025 (616923) 616923..617801 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
NP429_RS03030 (617791) 617791..618570 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NP429_RS03035 (618581) 618581..619054 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NP429_RS03040 (619077) 619077..620357 - 1281 WP_000681926.1 tagatose-bisphosphate aldolase subunit KbaZ -
NP429_RS03045 (620606) 620606..621415 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NP429_RS03050 (621470) 621470..621934 - 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NP429_RS03055 (621934) 621934..622269 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NP429_RS03060 (622418) 622418..623989 - 1572 WP_001273940.1 galactarate dehydratase -
NP429_RS03065 (624364) 624364..625698 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
NP429_RS03070 (625714) 625714..626484 + 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T252986 WP_000347252.1 NZ_CP101925:c621934-621470 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT252986 WP_001296435.1 NZ_CP101925:c622269-621934 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8FDB4

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References