Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3571307..3572001 | Replicon | chromosome |
| Accession | NZ_CP101922 | ||
| Organism | Escherichia coli strain JCC-EE 7 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | NP442_RS17565 | Protein ID | WP_001263493.1 |
| Coordinates | 3571307..3571705 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | NP442_RS17570 | Protein ID | WP_000554757.1 |
| Coordinates | 3571708..3572001 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3566967) | 3566967..3567047 | - | 81 | NuclAT_11 | - | - |
| - (3566967) | 3566967..3567047 | - | 81 | NuclAT_11 | - | - |
| - (3566967) | 3566967..3567047 | - | 81 | NuclAT_11 | - | - |
| - (3566967) | 3566967..3567047 | - | 81 | NuclAT_11 | - | - |
| NP442_RS17535 (3566307) | 3566307..3567551 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| NP442_RS17540 (3567643) | 3567643..3568101 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| NP442_RS17545 (3568362) | 3568362..3569819 | + | 1458 | WP_001293002.1 | cytosol nonspecific dipeptidase | - |
| NP442_RS17550 (3569876) | 3569876..3570397 | - | 522 | Protein_3431 | peptide chain release factor H | - |
| NP442_RS17555 (3570396) | 3570396..3570599 | - | 204 | Protein_3432 | RtcB family protein | - |
| NP442_RS17560 (3570845) | 3570845..3571297 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| NP442_RS17565 (3571307) | 3571307..3571705 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| NP442_RS17570 (3571708) | 3571708..3572001 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| NP442_RS17575 (3572053) | 3572053..3573108 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| NP442_RS17580 (3573179) | 3573179..3573964 | - | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| NP442_RS17585 (3573936) | 3573936..3575648 | + | 1713 | Protein_3438 | flagellar biosynthesis protein FlhA | - |
| NP442_RS17590 (3575872) | 3575872..3576369 | - | 498 | WP_000006242.1 | REP-associated tyrosine transposase RayT | - |
| NP442_RS17595 (3576545) | 3576545..3576712 | - | 168 | Protein_3440 | C40 family peptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T252980 WP_001263493.1 NZ_CP101922:c3571705-3571307 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T252980 NZ_CP101922:c3571705-3571307 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT252980 WP_000554757.1 NZ_CP101922:c3572001-3571708 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT252980 NZ_CP101922:c3572001-3571708 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|