Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2577685..2578044 Replicon chromosome
Accession NZ_CP101921
Organism Escherichia coli strain QA-1986 83

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag NP438_RS12435 Protein ID WP_001317028.1
Coordinates 2577685..2577879 (+) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2577878..2578044 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP438_RS12410 (2573383) 2573383..2573604 + 222 WP_000560224.1 killing protein KilR -
NP438_RS12415 (2573604) 2573604..2573774 + 171 WP_001352098.1 YdaE family protein -
NP438_RS12420 (2573849) 2573849..2574124 + 276 WP_024251429.1 hypothetical protein -
NP438_RS12425 (2574226) 2574226..2576826 + 2601 WP_000105136.1 exodeoxyribonuclease VIII -
NP438_RS12430 (2576819) 2576819..2577628 + 810 WP_000166319.1 recombination protein RecT -
NP438_RS12435 (2577685) 2577685..2577879 + 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
- (2577878) 2577878..2578044 - 167 NuclAT_1 - Antitoxin
- (2577878) 2577878..2578044 - 167 NuclAT_1 - Antitoxin
- (2577878) 2577878..2578044 - 167 NuclAT_1 - Antitoxin
- (2577878) 2577878..2578044 - 167 NuclAT_1 - Antitoxin
NP438_RS12440 (2577872) 2577872..2578069 + 198 WP_024170988.1 DUF1187 family protein -
NP438_RS12445 (2578148) 2578148..2578363 + 216 WP_000079604.1 excisionase XisR -
NP438_RS12450 (2578365) 2578365..2579600 + 1236 WP_000040852.1 site-specific integrase -
NP438_RS12455 (2579652) 2579652..2580587 + 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
NP438_RS12460 (2580716) 2580716..2582089 - 1374 WP_000123739.1 ATP-dependent RNA helicase DbpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2530502..2579600 49098


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T252957 WP_001317028.1 NZ_CP101921:2577685-2577879 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

Antitoxin


Download         Length: 167 bp

>AT252957 NZ_CP101921:c2578044-2577878 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References