Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3680036..3680715 Replicon chromosome
Accession NZ_CP101920
Organism Escherichia coli strain QA-1986 630

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag NP447_RS17995 Protein ID WP_000057540.1
Coordinates 3680413..3680715 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NP447_RS17990 Protein ID WP_000806442.1
Coordinates 3680036..3680377 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP447_RS17980 (3676280) 3676280..3677212 - 933 WP_023155692.1 glutaminase A -
NP447_RS17985 (3677474) 3677474..3679978 + 2505 WP_000083962.1 copper-exporting P-type ATPase CopA -
NP447_RS17990 (3680036) 3680036..3680377 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NP447_RS17995 (3680413) 3680413..3680715 - 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NP447_RS18000 (3680848) 3680848..3681642 + 795 WP_000365180.1 TraB/GumN family protein -
NP447_RS18005 (3681846) 3681846..3682325 + 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NP447_RS18010 (3682362) 3682362..3684014 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
NP447_RS18015 (3684232) 3684232..3685452 + 1221 WP_001251608.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T252939 WP_000057540.1 NZ_CP101920:c3680715-3680413 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT252939 WP_000806442.1 NZ_CP101920:c3680377-3680036 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References