Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3655877..3656418 Replicon chromosome
Accession NZ_CP101915
Organism Escherichia coli strain Evo1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NP443_RS17820 Protein ID WP_000615983.1
Coordinates 3656140..3656418 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NP443_RS17815 Protein ID WP_000729703.1
Coordinates 3655877..3656137 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP443_RS17795 (3651552) 3651552..3652337 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NP443_RS17800 (3652309) 3652309..3654021 + 1713 Protein_3481 flagellar biosynthesis protein FlhA -
NP443_RS17805 (3654245) 3654245..3654742 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NP443_RS17810 (3654918) 3654918..3655667 - 750 WP_000056849.1 C40 family peptidase -
NP443_RS17815 (3655877) 3655877..3656137 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NP443_RS17820 (3656140) 3656140..3656418 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NP443_RS17825 (3656574) 3656574..3657314 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NP443_RS17830 (3657285) 3657285..3658052 - 768 WP_000333380.1 class II glutamine amidotransferase -
NP443_RS17835 (3658258) 3658258..3658836 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3649680..3664960 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T252919 WP_000615983.1 NZ_CP101915:3656140-3656418 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT252919 WP_000729703.1 NZ_CP101915:3655877-3656137 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References