Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3649680..3650374 Replicon chromosome
Accession NZ_CP101915
Organism Escherichia coli strain Evo1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NP443_RS17780 Protein ID WP_001263489.1
Coordinates 3649680..3650078 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NP443_RS17785 Protein ID WP_000554758.1
Coordinates 3650081..3650374 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3645268) 3645268..3645348 - 81 NuclAT_10 - -
- (3645268) 3645268..3645348 - 81 NuclAT_10 - -
- (3645268) 3645268..3645348 - 81 NuclAT_10 - -
- (3645268) 3645268..3645348 - 81 NuclAT_10 - -
NP443_RS17755 (3645944) 3645944..3646402 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NP443_RS17760 (3646663) 3646663..3648120 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NP443_RS17765 (3648177) 3648177..3648698 - 522 Protein_3474 peptide chain release factor H -
NP443_RS17770 (3648694) 3648694..3648900 - 207 Protein_3475 RtcB family protein -
NP443_RS17775 (3649218) 3649218..3649670 - 453 WP_074514769.1 GNAT family N-acetyltransferase -
NP443_RS17780 (3649680) 3649680..3650078 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NP443_RS17785 (3650081) 3650081..3650374 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NP443_RS17790 (3650426) 3650426..3651481 - 1056 WP_001226164.1 DNA polymerase IV -
NP443_RS17795 (3651552) 3651552..3652337 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NP443_RS17800 (3652309) 3652309..3654021 + 1713 Protein_3481 flagellar biosynthesis protein FlhA -
NP443_RS17805 (3654245) 3654245..3654742 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3649680..3664960 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T252918 WP_001263489.1 NZ_CP101915:c3650078-3649680 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT252918 WP_000554758.1 NZ_CP101915:c3650374-3650081 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References