Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3225020..3225622 Replicon chromosome
Accession NZ_CP101858
Organism Escherichia coli strain LP50-1

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NPX80_RS15660 Protein ID WP_000897305.1
Coordinates 3225020..3225331 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NPX80_RS15665 Protein ID WP_000356397.1
Coordinates 3225332..3225622 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX80_RS15630 (3220050) 3220050..3220835 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
NPX80_RS15635 (3220934) 3220934..3221533 + 600 WP_001295269.1 glucose-1-phosphatase -
NPX80_RS15640 (3221527) 3221527..3222399 + 873 WP_000920762.1 virulence factor BrkB family protein -
NPX80_RS15645 (3222396) 3222396..3222833 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NPX80_RS15650 (3222878) 3222878..3223819 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NPX80_RS15655 (3223883) 3223883..3224791 - 909 WP_001162704.1 alpha/beta hydrolase -
NPX80_RS15660 (3225020) 3225020..3225331 + 312 WP_000897305.1 hypothetical protein Toxin
NPX80_RS15665 (3225332) 3225332..3225622 + 291 WP_000356397.1 NadS family protein Antitoxin
NPX80_RS15670 (3226227) 3226227..3226445 + 219 WP_001315930.1 CopG family transcriptional regulator -
NPX80_RS15675 (3226665) 3226665..3226907 + 243 WP_001087409.1 protein YiiF -
NPX80_RS15680 (3227237) 3227237..3228166 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
NPX80_RS15685 (3228163) 3228163..3228798 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NPX80_RS15690 (3228795) 3228795..3229697 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T252788 WP_000897305.1 NZ_CP101858:3225020-3225331 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT252788 WP_000356397.1 NZ_CP101858:3225332-3225622 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References