Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2422660..2423459 Replicon chromosome
Accession NZ_CP101858
Organism Escherichia coli strain LP50-1

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag NPX80_RS11770 Protein ID WP_000347266.1
Coordinates 2422995..2423459 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NPX80_RS11765 Protein ID WP_001307405.1
Coordinates 2422660..2422995 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX80_RS11750 (2418445) 2418445..2419215 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NPX80_RS11755 (2419231) 2419231..2420565 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NPX80_RS11760 (2420940) 2420940..2422511 + 1572 WP_001273741.1 galactarate dehydratase -
NPX80_RS11765 (2422660) 2422660..2422995 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NPX80_RS11770 (2422995) 2422995..2423459 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NPX80_RS11775 (2423514) 2423514..2424323 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NPX80_RS11780 (2424572) 2424572..2425852 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
NPX80_RS11785 (2425875) 2425875..2426348 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NPX80_RS11790 (2426359) 2426359..2427138 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NPX80_RS11795 (2427128) 2427128..2428006 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
NPX80_RS11800 (2428024) 2428024..2428458 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2410393..2423459 13066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T252785 WP_000347266.1 NZ_CP101858:2422995-2423459 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT252785 WP_001307405.1 NZ_CP101858:2422660-2422995 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References