Detailed information of TA system TA252686

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34892..35318
• Replicon: plasmid unnamed6
• Accession: NZ_CP101782
• Organism: Klebsiella pneumoniae strain hvKP340

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: NPJ84_RS29355
• Protein ID: WP_001372321.1
• Coordinates: 35193..35318 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34892..35116 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NPJ84_RS29320 (30002)	30002..30457	-	456	Protein_39	hypothetical protein	-
NPJ84_RS29325 (30759)	30759..31286	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
NPJ84_RS29330 (31344)	31344..31577	+	234	WP_000006003.1	DUF905 family protein	-
NPJ84_RS29335 (31638)	31638..33661	+	2024	Protein_42	ParB/RepB/Spo0J family partition protein	-
NPJ84_RS29340 (33730)	33730..34164	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
NPJ84_RS29345 (34161)	34161..34880	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
- (34892)	34892..35116	+	225	NuclAT_0	-	Antitoxin
- (34892)	34892..35116	+	225	NuclAT_0	-	Antitoxin
- (34892)	34892..35116	+	225	NuclAT_0	-	Antitoxin
- (34892)	34892..35116	+	225	NuclAT_0	-	Antitoxin
NPJ84_RS29350 (35102)	35102..35251	+	150	Protein_45	plasmid maintenance protein Mok	-
NPJ84_RS29355 (35193)	35193..35318	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
NPJ84_RS29360 (35637)	35637..35933	-	297	Protein_47	hypothetical protein	-
NPJ84_RS29365 (36233)	36233..36529	+	297	WP_001272251.1	hypothetical protein	-
NPJ84_RS29370 (36640)	36640..37461	+	822	WP_001234445.1	DUF932 domain-containing protein	-
NPJ84_RS29375 (37758)	37758..38405	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
NPJ84_RS29380 (38682)	38682..39065	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
NPJ84_RS29385 (39346)	39346..40050	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..111949	111949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T252686 WP_001372321.1 NZ_CP101782:35193-35318 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT252686 NZ_CP101782:34892-35116 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin