Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 5492224..5492951 | Replicon | chromosome |
| Accession | NZ_CP101776 | ||
| Organism | Klebsiella pneumoniae strain hvKP340 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | NPJ84_RS27090 | Protein ID | WP_011251285.1 |
| Coordinates | 5492224..5492535 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | NPJ84_RS27095 | Protein ID | WP_011251286.1 |
| Coordinates | 5492532..5492951 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NPJ84_RS27065 (NPJ84_27065) | 5487742..5488236 | - | 495 | WP_004212794.1 | thermonuclease family protein | - |
| NPJ84_RS27070 (NPJ84_27070) | 5488414..5488680 | + | 267 | WP_223175074.1 | DUF1173 family protein | - |
| NPJ84_RS27075 (NPJ84_27075) | 5488713..5489363 | + | 651 | WP_068893702.1 | DUF1173 family protein | - |
| NPJ84_RS27080 (NPJ84_27080) | 5490718..5491449 | - | 732 | WP_256556261.1 | IS1-like element IS1D family transposase | - |
| NPJ84_RS27085 (NPJ84_27085) | 5491584..5492019 | + | 436 | Protein_5295 | DDE-type integrase/transposase/recombinase | - |
| NPJ84_RS27090 (NPJ84_27090) | 5492224..5492535 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| NPJ84_RS27095 (NPJ84_27095) | 5492532..5492951 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| NPJ84_RS27100 (NPJ84_27100) | 5493098..5494066 | + | 969 | WP_086642568.1 | IS5 family transposase | - |
| NPJ84_RS27105 (NPJ84_27105) | 5494138..5494503 | - | 366 | WP_048333448.1 | hypothetical protein | - |
| NPJ84_RS27110 (NPJ84_27110) | 5494517..5495305 | - | 789 | WP_040217257.1 | hypothetical protein | - |
| NPJ84_RS27115 (NPJ84_27115) | 5495326..5495946 | - | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| NPJ84_RS27120 (NPJ84_27120) | 5496365..5497000 | + | 636 | WP_223171879.1 | hypothetical protein | - |
| NPJ84_RS27125 (NPJ84_27125) | 5497313..5497783 | + | 471 | WP_048333570.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | iroN / iucA / iucB / iucC / iucD / iutA | 5386384..5609234 | 222850 | |
| - | inside | IScluster/Tn | - | - | 5476725..5500857 | 24132 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T252681 WP_011251285.1 NZ_CP101776:5492224-5492535 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT252681 WP_011251286.1 NZ_CP101776:5492532-5492951 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|