Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 29846..30573 Replicon plasmid unnamed4
Accession NZ_CP101774
Organism Klebsiella pneumoniae strain hvKP323

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag NO368_RS27695 Protein ID WP_011251285.1
Coordinates 29846..30157 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NO368_RS27700 Protein ID WP_011251286.1
Coordinates 30154..30573 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO368_RS27670 (NO368_27670) 25365..25859 - 495 WP_004212794.1 thermonuclease family protein -
NO368_RS27675 (NO368_27675) 26037..26303 + 267 WP_223175074.1 DUF1173 family protein -
NO368_RS27680 (NO368_27680) 26336..26986 + 651 WP_068893702.1 DUF1173 family protein -
NO368_RS27690 (NO368_27690) 29204..29641 + 438 Protein_33 DDE-type integrase/transposase/recombinase -
NO368_RS27695 (NO368_27695) 29846..30157 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
NO368_RS27700 (NO368_27700) 30154..30573 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
NO368_RS27705 (NO368_27705) 30720..31688 + 969 WP_086642568.1 IS5 family transposase -
NO368_RS27710 (NO368_27710) 31760..32125 - 366 WP_048333448.1 hypothetical protein -
NO368_RS27715 (NO368_27715) 32139..32927 - 789 WP_040217257.1 hypothetical protein -
NO368_RS27720 (NO368_27720) 32948..33568 - 621 WP_223175004.1 conjugative transfer protein MobI(A/C) -
NO368_RS27725 (NO368_27725) 33987..34622 + 636 WP_223171879.1 hypothetical protein -
NO368_RS27730 (NO368_27730) 34935..35405 + 471 WP_048333570.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / iucA / iucB / iucC / iucD / iutA / rmpA2 1..190026 190026
- inside IScluster/Tn - - 13390..38413 25023


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T252658 WP_011251285.1 NZ_CP101774:29846-30157 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT252658 WP_011251286.1 NZ_CP101774:30154-30573 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References