Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1187129..1187856 Replicon chromosome
Accession NZ_CP101705
Organism Escherichia coli strain 035152

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag NPL72_RS05830 Protein ID WP_000547564.1
Coordinates 1187129..1187440 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NPL72_RS05835 Protein ID WP_000126294.1
Coordinates 1187437..1187856 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPL72_RS05800 (1182271) 1182271..1183980 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
NPL72_RS05805 (1183990) 1183990..1184532 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
NPL72_RS05810 (1184532) 1184532..1185299 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NPL72_RS05815 (1185296) 1185296..1185706 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NPL72_RS05820 (1185699) 1185699..1186169 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NPL72_RS05825 (1186194) 1186194..1186955 + 762 WP_001026446.1 hypothetical protein -
NPL72_RS05830 (1187129) 1187129..1187440 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
NPL72_RS05835 (1187437) 1187437..1187856 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NPL72_RS05840 (1187970) 1187970..1189394 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
NPL72_RS05845 (1189403) 1189403..1190860 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NPL72_RS05850 (1191120) 1191120..1192130 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
NPL72_RS05855 (1192279) 1192279..1192806 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T252531 WP_000547564.1 NZ_CP101705:1187129-1187440 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT252531 WP_000126294.1 NZ_CP101705:1187437-1187856 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References