Detailed information of TA system TA025250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 581040..581693
• Replicon: chromosome
• Accession: NC_015566
• Organism: Serratia sp. AS12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U3YYL4
• Locus tag: SERAS12_RS02685
• Protein ID: WP_013811355.1
• Coordinates: 581040..581390 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SERAS12_RS02690
• Protein ID: WP_013811356.1
• Coordinates: 581394..581693 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SERAS12_RS02660	576519..577610	+	1092	WP_004949798.1	dimethyl sulfone monooxygenase SfnG	-
SERAS12_RS02665	577612..578313	-	702	WP_006323392.1	trehalose-6-phosphate synthase	-
SERAS12_RS02670	578328..578867	-	540	WP_013811352.1	GNAT family N-acetyltransferase	-
SERAS12_RS02675	578925..580304	-	1380	WP_013811353.1	glutamine synthetase family protein	-
SERAS12_RS02680	580560..580874	+	315	WP_013811354.1	hypothetical protein	-
SERAS12_RS02685	581040..581390	+	351	WP_013811355.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SERAS12_RS02690	581394..581693	+	300	WP_013811356.1	helix-turn-helix transcriptional regulator	Antitoxin
SERAS12_RS02695	581786..582862	-	1077	WP_013811357.1	YncE family protein	-
SERAS12_RS02700	583148..584416	-	1269	WP_013811358.1	multidrug effflux MFS transporter	-
SERAS12_RS02705	584416..585639	-	1224	WP_013811359.1	cytochrome P450	-
SERAS12_RS02710	585654..585911	-	258	WP_013811360.1	acyl carrier protein	-
SERAS12_RS02715	585958..586509	-	552	WP_013811361.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13456.56 Da
• Isoelectric Point: 8.5393

>T25250 WP_013811355.1 NC_015566:581040-581390 [Serratia sp. AS12]
MWTVITTEEFDRWFAVQDELTQEKVLAALVLLERSGPNLSRPFVDSLRGSQHPNMKELRVQHKGRPIRAFFAFDSLRQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQHLMCNFKE

Nucleotide

• Length: 351 bp

>T25250 NC_015566:581040-581390 [Serratia sp. AS12]
ATGTGGACTGTAATAACAACGGAAGAGTTTGATCGGTGGTTTGCGGTGCAGGATGAACTTACTCAGGAGAAAGTGCTGGC
TGCATTAGTATTGCTTGAGCGGAGTGGCCCCAACCTCTCACGTCCTTTTGTAGACTCGTTGAGAGGGTCACAGCACCCTA
ATATGAAAGAACTGCGGGTTCAGCATAAAGGGCGGCCGATTCGCGCTTTTTTTGCCTTTGACTCACTACGTCAGGCTATT
GTTTTGTGTGCCGGCGATAAGACTGGTAACGAGAAGCGGTTTTACAAAGTCATGCTGCCTATTGCGGATGCGCAGTTTAC
CCAGCATTTGATGTGTAATTTTAAGGAGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11183.85 Da
• Isoelectric Point: 5.1667

>AT25250 WP_013811356.1 NC_015566:581394-581693 [Serratia sp. AS12]
MGTLKDMLASRTPESLQRIEEMAQEMWLEARLYQLREALNISQQELANNLGISQPSVAAIEQRGNELKISTLKRYVEAMG
GTLRLDVELPDGKHVGFRV

Nucleotide

• Length: 300 bp

>AT25250 NC_015566:581394-581693 [Serratia sp. AS12]
ATGGGTACTTTAAAAGACATGCTAGCCTCGCGTACCCCGGAGAGTCTGCAACGAATCGAAGAAATGGCGCAAGAGATGTG
GCTGGAAGCACGGTTGTACCAATTGCGTGAGGCGCTGAATATCTCACAGCAGGAACTGGCGAATAATCTGGGTATCTCCC
AGCCTTCCGTCGCGGCGATAGAGCAGCGTGGCAATGAGCTGAAGATATCGACATTGAAGCGCTATGTGGAAGCGATGGGC
GGAACGTTGCGGCTGGATGTCGAACTGCCGGACGGCAAGCACGTTGGATTTAGAGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U3YYL4