Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3562055..3562854 Replicon chromosome
Accession NZ_CP101666
Organism Escherichia coli strain M171-1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NO473_RS17335 Protein ID WP_000347273.1
Coordinates 3562055..3562519 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NO473_RS17340 Protein ID WP_001307405.1
Coordinates 3562519..3562854 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO473_RS17305 (3557056) 3557056..3557490 - 435 WP_000948842.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NO473_RS17310 (3557508) 3557508..3558386 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NO473_RS17315 (3558376) 3558376..3559155 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
NO473_RS17320 (3559166) 3559166..3559639 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
NO473_RS17325 (3559662) 3559662..3560942 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
NO473_RS17330 (3561191) 3561191..3562000 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NO473_RS17335 (3562055) 3562055..3562519 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NO473_RS17340 (3562519) 3562519..3562854 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NO473_RS17345 (3563003) 3563003..3564574 - 1572 WP_001273753.1 galactarate dehydratase -
NO473_RS17350 (3564949) 3564949..3566283 + 1335 WP_130062351.1 galactarate/glucarate/glycerate transporter GarP -
NO473_RS17355 (3566299) 3566299..3567069 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T252470 WP_000347273.1 NZ_CP101666:c3562519-3562055 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT252470 WP_001307405.1 NZ_CP101666:c3562854-3562519 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References