Detailed information of TA system TA025243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5971531..5972205
• Replicon: chromosome
• Accession: NC_015563
• Organism: Delftia sp. Cs1-4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: F6AYN4
• Locus tag: DELCS14_RS26210
• Protein ID: WP_013804307.1
• Coordinates: 5971531..5971812 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: DELCS14_RS26215
• Protein ID: WP_012203141.1
• Coordinates: 5971894..5972205 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DELCS14_RS26190	5967197..5967763	-	567	WP_013804303.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
DELCS14_RS26195	5967769..5968797	-	1029	WP_013804304.1	type I-F CRISPR-associated protein Csy3	-
DELCS14_RS26200	5968852..5969970	-	1119	WP_013804305.1	type I-F CRISPR-associated protein Csy2	-
DELCS14_RS26205	5969967..5971364	-	1398	WP_013804306.1	type I-F CRISPR-associated protein Csy1	-
DELCS14_RS26210	5971531..5971812	+	282	WP_013804307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DELCS14_RS26215	5971894..5972205	+	312	WP_012203141.1	HigA family addiction module antidote protein	Antitoxin
DELCS14_RS26220	5972209..5975697	-	3489	WP_013804308.1	type I-F CRISPR-associated helicase Cas3	-
DELCS14_RS26225	5975694..5976716	-	1023	WP_013804309.1	type I-F CRISPR-associated endonuclease Cas1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10747.38 Da
• Isoelectric Point: 8.5442

>T25243 WP_013804307.1 NC_015563:5971531-5971812 [Delftia sp. Cs1-4]
MAITSFKCPETQRLFTIGKTRRFANIQSVAERKLAQLDAAVTLEFLRSPPGNRLEMLVGDRQGQHSIRINDQWRLCFVWT
EKGVVDVEIVDYH

Nucleotide

• Length: 282 bp

>T25243 NC_015563:5971531-5971812 [Delftia sp. Cs1-4]
ATGGCGATCACTTCCTTCAAGTGCCCGGAAACCCAGCGCCTGTTCACCATCGGCAAGACCCGCCGGTTCGCCAACATCCA
GAGCGTGGCGGAGCGCAAGCTGGCTCAACTGGATGCGGCTGTGACACTTGAATTTCTGCGCAGCCCTCCTGGCAATCGCC
TGGAAATGCTCGTGGGAGATCGGCAGGGCCAGCACAGCATCCGCATCAACGACCAGTGGCGCCTCTGCTTTGTCTGGACG
GAAAAGGGAGTGGTTGACGTAGAGATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11615.32 Da
• Isoelectric Point: 8.6927

>AT25243 WP_012203141.1 NC_015563:5971894-5972205 [Delftia sp. Cs1-4]
MLKNGMRPIHPGEVLREDYLKPMDMSANALARHLHVPASRVNDIVLERRGITADTALRLSRFFGGDAQSWLNLQTAYDLR
TAELNAELQASVRQVHPMQLHTA

Nucleotide

• Length: 312 bp

>AT25243 NC_015563:5971894-5972205 [Delftia sp. Cs1-4]
ATGCTTAAAAACGGCATGCGCCCCATACACCCAGGTGAAGTCCTGCGCGAGGACTACCTGAAGCCCATGGACATGAGCGC
CAACGCGCTGGCCAGGCACCTGCACGTTCCCGCCTCGCGCGTGAACGACATCGTGCTGGAGCGGCGCGGCATCACGGCCG
ACACGGCGCTGCGGCTGAGCCGCTTCTTTGGGGGCGACGCTCAGTCCTGGCTGAACCTTCAGACCGCCTACGACCTGCGC
ACGGCAGAGCTGAACGCGGAGTTGCAGGCGTCCGTGAGGCAAGTTCATCCGATGCAGCTGCATACCGCCTGA

Structures

Toxin

• AlphaFold DB: F6AYN4