Detailed information of TA system TA025241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3514780..3515373
• Replicon: chromosome
• Accession: NC_015563
• Organism: Delftia sp. Cs1-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6ASE3
• Locus tag: DELCS14_RS15430
• Protein ID: WP_013802475.1
• Coordinates: 3515095..3515373 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DELCS14_RS15425
• Protein ID: WP_013802474.1
• Coordinates: 3514780..3515082 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DELCS14_RS15405	3510584..3511858	+	1275	WP_013802470.1	serine hydroxymethyltransferase	-
DELCS14_RS15410	3511951..3513003	-	1053	WP_013802471.1	hypothetical protein	-
DELCS14_RS15415	3513000..3513473	-	474	WP_013802472.1	hypothetical protein	-
DELCS14_RS15420	3513625..3514665	-	1041	WP_013802473.1	patatin-like phospholipase family protein	-
DELCS14_RS15425	3514780..3515082	-	303	WP_013802474.1	HigA family addiction module antidote protein	Antitoxin
DELCS14_RS15430	3515095..3515373	-	279	WP_013802475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DELCS14_RS15435	3515440..3516342	-	903	WP_013802476.1	LysR family transcriptional regulator	-
DELCS14_RS15440	3516357..3516893	-	537	WP_013802477.1	MarR family winged helix-turn-helix transcriptional regulator	-
DELCS14_RS15445	3517098..3517847	+	750	Protein_3113	FAD-dependent monooxygenase	-
DELCS14_RS15450	3517905..3519176	+	1272	WP_013801385.1	IS256 family transposase	-
DELCS14_RS30000	3519268..3520009	-	742	Protein_3115	IS5 family transposase	-
DELCS14_RS29530	3520058..3520321	-	264	WP_013802480.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3517905..3519176	1271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10518.17 Da
• Isoelectric Point: 9.2618

>T25241 WP_013802475.1 NC_015563:c3515373-3515095 [Delftia sp. Cs1-4]
MILDFRCADTQALMLGKRVARFANLEAAAMRKLVQLNAATSLEFLRIPPGNRLEALQGSRRGQYSIRINEQWRLCFVWAD
GHASQVEIVDYH

Nucleotide

• Length: 279 bp

>T25241 NC_015563:c3515373-3515095 [Delftia sp. Cs1-4]
ATGATCCTCGATTTCAGATGTGCAGACACCCAGGCATTGATGCTTGGCAAGCGCGTTGCGCGGTTCGCCAACCTGGAGGC
CGCTGCCATGCGCAAGCTGGTGCAGCTGAACGCAGCGACCTCCCTGGAGTTCCTGCGCATTCCGCCAGGCAACAGACTGG
AGGCTCTGCAAGGAAGCCGCAGGGGCCAGTACAGCATTCGGATCAATGAGCAATGGCGCCTTTGCTTCGTCTGGGCAGAT
GGCCATGCCAGCCAGGTCGAGATCGTGGATTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11364.06 Da
• Isoelectric Point: 5.1007

>AT25241 WP_013802474.1 NC_015563:c3515082-3514780 [Delftia sp. Cs1-4]
MTREVKWPHPGEILMQEWLEPMGLSQYALAKAIDVPPRRINEIVKGMRGITADTALRLAVFFGTDAQSWLNLQSDYDLAE
ARDAMADVLARIQRFEPSHP

Nucleotide

• Length: 303 bp

>AT25241 NC_015563:c3515082-3514780 [Delftia sp. Cs1-4]
ATGACCCGCGAAGTCAAATGGCCGCACCCAGGAGAAATCCTGATGCAGGAGTGGCTCGAACCCATGGGCCTGAGCCAGTA
CGCGTTGGCAAAGGCCATCGATGTGCCGCCCCGGCGCATCAACGAGATCGTGAAGGGCATGCGCGGCATCACGGCGGATA
CGGCGCTGCGCCTGGCGGTCTTCTTTGGCACGGACGCGCAAAGCTGGCTCAACCTGCAGTCCGACTACGACCTTGCCGAA
GCCCGCGATGCCATGGCGGACGTGCTGGCGCGAATCCAGCGGTTTGAGCCCTCGCATCCCTGA

Structures

Toxin

• AlphaFold DB: A0A031I1T8