Detailed information of TA system TA025239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 298669..299292
• Replicon: chromosome
• Accession: NC_015563
• Organism: Delftia sp. Cs1-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6AKU7
• Locus tag: DELCS14_RS01315
• Protein ID: WP_013800140.1
• Coordinates: 298669..298950 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DELCS14_RS01320
• Protein ID: WP_013800141.1
• Coordinates: 298993..299292 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DELCS14_RS01290	293903..294766	+	864	WP_012202199.1	branched-chain amino acid ABC transporter permease	-
DELCS14_RS01295	294767..295741	+	975	WP_013800136.1	branched-chain amino acid ABC transporter permease	-
DELCS14_RS01300	295728..296492	+	765	WP_013800137.1	ABC transporter ATP-binding protein	-
DELCS14_RS01305	296479..297186	+	708	WP_013800138.1	ABC transporter ATP-binding protein	-
DELCS14_RS01310	297364..298539	+	1176	WP_013800139.1	ABC transporter substrate-binding protein	-
DELCS14_RS01315	298669..298950	+	282	WP_013800140.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DELCS14_RS01320	298993..299292	+	300	WP_013800141.1	HigA family addiction module antidote protein	Antitoxin
DELCS14_RS01325	299385..300158	-	774	WP_012202205.1	SDR family oxidoreductase	-
DELCS14_RS01330	300292..301098	-	807	WP_012202206.1	IclR family transcriptional regulator	-
DELCS14_RS01335	301143..301916	-	774	WP_012202207.1	ferredoxin--NADP reductase	-
DELCS14_RS01340	302177..303112	-	936	WP_013800142.1	LysR family transcriptional regulator	-
DELCS14_RS01345	303205..303996	+	792	WP_013800143.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11067.70 Da
• Isoelectric Point: 9.3577

>T25239 WP_013800140.1 NC_015563:298669-298950 [Delftia sp. Cs1-4]
MTIQSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSELEIVDYH

Nucleotide

• Length: 282 bp

>T25239 NC_015563:298669-298950 [Delftia sp. Cs1-4]
ATGACGATCCAGTCATTCAAGTGCCCCGACACCGAGCAACTGTTCCAGCAGAGGCGGGTGCGTCGGTGGAGCAGCATTGA
AGTGGTCGCGCTGCGCAAGCTTCGGATGCTGCATGCCGCTCATGTGCTTCAGGACTTGAGGGTCCCGCCCGGAAACCGGC
TGGAGGCGCTGCACGGAGATCGCAAGGGGCAGCACAGCATCCGCATCAATGACCAGTGGCGCGTGTGCTTTGTGTGGACC
CATGAAGGCCCGAGTGAGCTGGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10990.57 Da
• Isoelectric Point: 4.5183

>AT25239 WP_013800141.1 NC_015563:298993-299292 [Delftia sp. Cs1-4]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADI
AREAIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT25239 NC_015563:298993-299292 [Delftia sp. Cs1-4]
ATGAACAAACCTTTGAACCTCCCCACGCCAGGCGAAATCCTGGCCACTGAATTCCTGGAGCCCATGGGCCTGACGCAATA
CCGCCTGGCCCAGGCCATCCATGTCCCGCAGACACGCATCGCGGCCATCGTCAAGAACGGCCGCTCCATCACCGCGGATA
CGGCGCTGCGCCTTGCGCGCTTCTTTGGCACGTCCGTGGAGTTCTGGATGAATCTGCAAAGCCAGTACGACGCTGATATC
GCGCGCGAGGCCATAGGCGACGATCTGGAGTCCATCGAGCCCTTTGCCCTGCAGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A5P8XEH0