Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1112713..1113440 Replicon chromosome
Accession NZ_CP101615
Organism Escherichia coli strain DC71

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag NOF56_RS05425 Protein ID WP_000547564.1
Coordinates 1112713..1113024 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NOF56_RS05430 Protein ID WP_000126294.1
Coordinates 1113021..1113440 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOF56_RS05395 (1107855) 1107855..1109564 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
NOF56_RS05400 (1109574) 1109574..1110116 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
NOF56_RS05405 (1110116) 1110116..1110883 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NOF56_RS05410 (1110880) 1110880..1111290 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NOF56_RS05415 (1111283) 1111283..1111753 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
NOF56_RS05420 (1111778) 1111778..1112539 + 762 WP_001026446.1 hypothetical protein -
NOF56_RS05425 (1112713) 1112713..1113024 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
NOF56_RS05430 (1113021) 1113021..1113440 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NOF56_RS05435 (1113554) 1113554..1114978 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
NOF56_RS05440 (1114987) 1114987..1116444 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NOF56_RS05445 (1116704) 1116704..1117714 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
NOF56_RS05450 (1117863) 1117863..1118390 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T252386 WP_000547564.1 NZ_CP101615:1112713-1113024 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT252386 WP_000126294.1 NZ_CP101615:1113021-1113440 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References