Detailed information of TA system TA025222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3002502..3003092
• Replicon: chromosome
• Accession: NC_015510
• Organism: Haliscomenobacter hydrossis DSM 1100

Toxin (Protein)

• Gene name: graT
• Uniprot ID: F4KV76
• Locus tag: HALHY_RS11675
• Protein ID: WP_013764752.1
• Coordinates: 3002502..3002783 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HALHY_RS11680
• Protein ID: WP_013764753.1
• Coordinates: 3002793..3003092 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HALHY_RS11655	2998003..2998764	+	762	WP_013764747.1	LytTR family DNA-binding domain-containing protein	-
HALHY_RS11660	2998833..2999567	+	735	WP_013764748.1	DUF2490 domain-containing protein	-
HALHY_RS11665	2999774..3000355	+	582	WP_013764749.1	OmpH family outer membrane protein	-
HALHY_RS11670	3000483..3002321	+	1839	WP_013764750.1	phosphoenolpyruvate carboxykinase (GTP)	-
HALHY_RS11675	3002502..3002783	+	282	WP_013764752.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HALHY_RS11680	3002793..3003092	+	300	WP_013764753.1	HigA family addiction module antidote protein	Antitoxin
HALHY_RS11685	3003224..3004222	+	999	WP_013764754.1	virulence RhuM family protein	-
HALHY_RS11690	3004361..3006190	+	1830	WP_044233652.1	ABC transporter ATP-binding protein/permease	-
HALHY_RS11700	3006536..3006751	+	216	WP_013764756.1	hypothetical protein	-
HALHY_RS11705	3006879..3007355	+	477	WP_044233654.1	hypothetical protein	-
HALHY_RS11710	3007376..3007603	-	228	WP_044233656.1	DUF433 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-79)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11227.87 Da
• Isoelectric Point: 9.9272

>T25222 WP_013764752.1 NC_015510:3002502-3002783 [Haliscomenobacter hydrossis DSM 1100]
MIKSFADKETEKIWNGSYSKKFPHDIQPVSRRKLRMINSAQDIEDLRIPPGNRLEKLKGDLKDFWSIRINNQWRIIFKWE
DNNALEVQIIDYH

Nucleotide

• Length: 282 bp

>T25222 NC_015510:3002502-3002783 [Haliscomenobacter hydrossis DSM 1100]
ATGATTAAAAGCTTTGCAGATAAGGAAACTGAAAAAATTTGGAATGGATCTTACTCCAAAAAGTTTCCCCATGACATCCA
ACCCGTGTCAAGAAGAAAGCTCAGAATGATCAACAGTGCACAAGACATTGAAGACTTACGAATACCTCCGGGAAATAGAT
TAGAAAAGCTGAAAGGAGACTTGAAGGATTTCTGGAGCATTCGAATCAACAACCAGTGGCGGATCATCTTCAAATGGGAA
GACAACAATGCACTGGAAGTCCAAATCATTGACTATCATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11285.15 Da
• Isoelectric Point: 5.7134

>AT25222 WP_013764753.1 NC_015510:3002793-3003092 [Haliscomenobacter hydrossis DSM 1100]
MEKLPNIHPGEILLEEFLLPMHISQYKLAKDLMIPQTRVSEIVKRKRRITADTALRLSKYFGTSAKFWLGLQDDYDLEES
GNLLAAELQAIPEAMPSIS

Nucleotide

• Length: 300 bp

>AT25222 NC_015510:3002793-3003092 [Haliscomenobacter hydrossis DSM 1100]
ATGGAAAAATTACCCAACATCCACCCCGGCGAGATTCTCCTTGAAGAATTCCTCCTCCCCATGCATATCAGTCAATACAA
ACTGGCCAAAGACCTGATGATTCCCCAAACGAGAGTCAGTGAAATTGTAAAAAGAAAACGGCGCATCACTGCTGATACTG
CTTTACGGTTGAGCAAATACTTTGGTACCTCGGCTAAATTTTGGCTTGGACTTCAAGATGATTATGATTTGGAGGAGTCT
GGAAATTTGCTGGCCGCAGAACTACAGGCTATTCCTGAGGCTATGCCTTCTATTTCTTAA

Structures

Toxin

• AlphaFold DB: F4KV76