Detailed information of TA system TA025217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2569898..2570486
• Replicon: chromosome
• Accession: NC_015460
• Organism: Gallibacterium anatis UMN179

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F4H9G9
• Locus tag: UMN179_RS12070
• Protein ID: WP_013747176.1
• Coordinates: 2570208..2570486 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: UMN179_RS12065
• Protein ID: WP_200909575.1
• Coordinates: 2569898..2570191 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMN179_RS12040	2565142..2565390	+	249	WP_013747172.1	hypothetical protein	-
UMN179_RS12045	2565446..2566744	+	1299	WP_052297113.1	tape measure domain-containing protein	-
UMN179_RS12050	2567747..2568859	+	1113	WP_080558821.1	phage tail tape measure protein	-
UMN179_RS12055	2568859..2569185	+	327	WP_013747173.1	phage tail protein	-
UMN179_RS12060	2569185..2569901	+	717	WP_013747174.1	phage minor tail protein L	-
UMN179_RS12065	2569898..2570191	-	294	WP_200909575.1	HigA family addiction module antidote protein	Antitoxin
UMN179_RS12070	2570208..2570486	-	279	WP_013747176.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
UMN179_RS12075	2570548..2571282	+	735	WP_013747177.1	C40 family peptidase	-
UMN179_RS12080	2571225..2571875	+	651	WP_043885300.1	tail assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2528672..2594330	65658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10466.96 Da
• Isoelectric Point: 7.0722

>T25217 WP_013747176.1 NC_015460:c2570486-2570208 [Gallibacterium anatis UMN179]
MIISFKHKGLQAFFETGSTAGIQPKHAQKLHLLLTTLNMVSDVAEMDMPGWNLHPLKGDLSGHWSVKVNANWRLTFKFEN
GDAEIVNYQDYH

Nucleotide

• Length: 279 bp

>T25217 NC_015460:c2570486-2570208 [Gallibacterium anatis UMN179]
ATGATTATTTCATTCAAACATAAAGGCTTGCAGGCATTTTTTGAAACTGGATCAACGGCAGGTATTCAACCTAAGCACGC
ACAAAAGCTCCATTTATTGCTTACTACCCTGAATATGGTTAGTGATGTTGCTGAAATGGATATGCCTGGTTGGAACTTGC
ATCCGCTGAAAGGGGATTTAAGCGGACATTGGTCCGTTAAAGTCAATGCCAACTGGCGTTTAACCTTTAAATTTGAAAAC
GGCGATGCTGAAATTGTCAATTATCAAGATTACCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11175.90 Da
• Isoelectric Point: 7.2017

>AT25217 WP_200909575.1 NC_015460:c2570191-2569898 [Gallibacterium anatis UMN179]
MFNPAHPGEVLRDIISEFKINEVADKLGVTRVTLSRILNAKTSVTPEMAVRLSKLLPNTSPNLWLNMQAQYDLWHLEQDK
QFDVQPLYANNPMDKRL

Nucleotide

• Length: 294 bp

>AT25217 NC_015460:c2570191-2569898 [Gallibacterium anatis UMN179]
ATGTTCAACCCAGCCCACCCCGGTGAAGTATTAAGAGATATTATTTCTGAATTTAAAATCAACGAGGTTGCCGATAAACT
TGGTGTAACACGCGTTACACTTTCCCGAATTTTAAATGCCAAAACGAGCGTTACCCCTGAAATGGCAGTGCGTCTTAGTA
AACTTTTGCCAAATACTTCACCGAACTTATGGCTAAATATGCAAGCTCAATATGACTTATGGCATTTAGAGCAAGATAAA
CAATTTGATGTTCAACCGTTATATGCGAACAATCCAATGGATAAACGCCTTTAA

Structures

Toxin

• AlphaFold DB: F4H9G9