Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 667646..668373 Replicon chromosome
Accession NZ_CP101513
Organism Escherichia coli strain HH194M

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MOO21_RS03255 Protein ID WP_000550189.1
Coordinates 667646..667960 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MOO21_RS03260 Protein ID WP_000560266.1
Coordinates 667957..668373 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO21_RS03235 (663813) 663813..664799 - 987 WP_023281494.1 Gfo/Idh/MocA family oxidoreductase -
MOO21_RS03240 (664878) 664878..665561 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
MOO21_RS03245 (665638) 665638..666141 - 504 WP_001295542.1 M48 family metallopeptidase -
MOO21_RS03250 (666226) 666226..667362 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MOO21_RS03255 (667646) 667646..667960 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MOO21_RS03260 (667957) 667957..668373 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MOO21_RS03265 (668418) 668418..670435 - 2018 Protein_639 NADPH-dependent 2,4-dienoyl-CoA reductase -
MOO21_RS03270 (670861) 670861..673212 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T252164 WP_000550189.1 NZ_CP101513:667646-667960 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT252164 WP_000560266.1 NZ_CP101513:667957-668373 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References