Detailed information of TA system TA025191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3200172..3200821
• Replicon: chromosome
• Accession: NC_015410
• Organism: Pseudomonas mendocina NK-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MDS_RS14720
• Protein ID: WP_041769503.1
• Coordinates: 3200172..3200516 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MDS_RS14725
• Protein ID: WP_013716000.1
• Coordinates: 3200513..3200821 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MDS_RS14705	3195937..3197790	+	1854	WP_013715996.1	asparagine synthase (glutamine-hydrolyzing)	-
MDS_RS14710	3197876..3199075	+	1200	WP_013715997.1	MFS transporter	-
MDS_RS14715	3199089..3200015	+	927	WP_013715998.1	LysR family transcriptional regulator	-
MDS_RS14720	3200172..3200516	+	345	WP_041769503.1	toxin	Toxin
MDS_RS14725	3200513..3200821	+	309	WP_013716000.1	transcriptional regulator	Antitoxin
MDS_RS25485	3201022..3201744	-	723	WP_080558760.1	peptidase E	-
MDS_RS25490	3201747..3202742	-	996	WP_080558761.1	ThiF family adenylyltransferase	-
MDS_RS25790	3202812..3203042	-	231	WP_148260819.1	hypothetical protein	-
MDS_RS14730	3203484..3205475	+	1992	WP_013716001.1	conjugal transfer protein TraG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3057752..3221063	163311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13346.21 Da
• Isoelectric Point: 10.1537

>T25191 WP_041769503.1 NC_015410:3200172-3200516 [Pseudomonas mendocina NK-01]
MNALFVELSPFERNRKAHLDDDEYSLFQQMLLANPEAGAVIANSGGLRKVRFGSVRRNKGKRGGVRVIYYYWHRGLQFWL
FTLYDKDELDDLSSDQRRQLKALLEREVKARQAP

Nucleotide

• Length: 345 bp

>T25191 NC_015410:3200172-3200516 [Pseudomonas mendocina NK-01]
ATGAATGCATTGTTCGTTGAGCTGTCACCCTTTGAGCGCAATCGAAAAGCCCACCTGGATGACGACGAGTACTCTCTATT
TCAGCAGATGCTCTTGGCCAACCCGGAGGCAGGCGCAGTCATCGCCAACAGCGGTGGTCTACGGAAGGTCCGTTTCGGAT
CGGTCAGGCGCAACAAGGGGAAGCGAGGTGGCGTTCGGGTGATTTACTACTACTGGCACCGCGGTCTGCAGTTCTGGCTC
TTCACCCTGTATGACAAAGACGAACTCGATGACCTGAGCAGTGACCAGCGCAGGCAGTTGAAAGCGCTCCTTGAGCGCGA
AGTGAAGGCGAGGCAAGCACCATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11521.27 Da
• Isoelectric Point: 9.7652

>AT25191 WP_013716000.1 NC_015410:3200513-3200821 [Pseudomonas mendocina NK-01]
MTKRDIFSELIEGFDDLAAAREGKRTLRTHKVEYTPIEPATGAEIVALREKLKMSQGVFAQAIHAKPATLKNWEQNRSTP
NDQATVLIRILSKHPELIRELA

Nucleotide

• Length: 309 bp

>AT25191 NC_015410:3200513-3200821 [Pseudomonas mendocina NK-01]
ATGACCAAACGCGATATTTTTTCCGAGCTGATCGAAGGCTTCGACGACCTGGCCGCAGCACGTGAAGGCAAGCGTACGCT
GCGTACCCACAAGGTCGAATACACGCCAATCGAGCCCGCGACAGGCGCGGAAATCGTCGCACTGCGCGAAAAGCTGAAGA
TGTCTCAGGGTGTATTCGCCCAGGCCATTCACGCCAAGCCAGCGACACTCAAGAACTGGGAACAGAACCGTTCAACGCCC
AACGATCAGGCTACCGTCTTGATCCGTATCCTGAGCAAGCATCCAGAACTCATACGCGAGCTGGCGTAG