Detailed information of TA system TA025190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3271792..3272378
• Replicon: chromosome
• Accession: NC_015388
• Organism: Desulfobacca acetoxidans DSM 11109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F2NIZ2
• Locus tag: DESAC_RS14780
• Protein ID: WP_013707866.1
• Coordinates: 3271792..3272070 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DESAC_RS14785
• Protein ID: WP_013707867.1
• Coordinates: 3272079..3272378 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DESAC_RS16360	3267342..3267512	-	171	WP_013707860.1	hypothetical protein	-
DESAC_RS14755	3268069..3268281	+	213	WP_013707861.1	type II toxin-antitoxin system HicB family antitoxin	-
DESAC_RS14760	3268345..3268581	+	237	WP_013707862.1	DUF2283 domain-containing protein	-
DESAC_RS14765	3269145..3269777	-	633	WP_013707863.1	PEP-CTERM sorting domain-containing protein	-
DESAC_RS14770	3269913..3270521	-	609	WP_013707864.1	hypothetical protein	-
DESAC_RS15545	3271102..3271767	+	666	WP_013707865.1	hypothetical protein	-
DESAC_RS14780	3271792..3272070	+	279	WP_013707866.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DESAC_RS14785	3272079..3272378	+	300	WP_013707867.1	HigA family addiction module antidote protein	Antitoxin
DESAC_RS14790	3272684..3272923	+	240	WP_013707868.1	type II toxin-antitoxin system HicB family antitoxin	-
DESAC_RS14795	3273121..3274050	-	930	WP_013707869.1	site-specific DNA-methyltransferase	-
DESAC_RS16145	3274371..3275309	-	939	WP_148231261.1	VWA domain-containing protein	-
DESAC_RS14805	3275261..3276349	-	1089	WP_041284019.1	ATP-binding protein	-
DESAC_RS14810	3276531..3277238	+	708	WP_013707870.1	potassium channel family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10464.86 Da
• Isoelectric Point: 8.1305

>T25190 WP_013707866.1 NC_015388:3271792-3272070 [Desulfobacca acetoxidans DSM 11109]
MIVSFKHKGLERYFLDGSVAGIQPKHAGRLRLILGRLHASSCAHDMNLPGLHLHELVGDRQDTWAVKVSGNWRLTFSFTG
QDAYNVNYEDYH

Nucleotide

• Length: 279 bp

>T25190 NC_015388:3271792-3272070 [Desulfobacca acetoxidans DSM 11109]
ATGATTGTCAGCTTCAAACACAAAGGCCTGGAACGTTATTTCCTTGATGGCAGCGTGGCCGGCATTCAGCCAAAACATGC
CGGAAGACTACGTCTCATCCTGGGCCGTCTCCATGCCTCCTCCTGTGCCCATGATATGAACCTTCCAGGTTTGCATCTGC
ATGAACTGGTTGGTGATCGTCAAGACACGTGGGCGGTAAAGGTAAGCGGCAACTGGAGGCTTACCTTTAGTTTTACCGGG
CAGGATGCCTATAACGTTAATTATGAAGATTATCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10816.66 Da
• Isoelectric Point: 8.0807

>AT25190 WP_013707867.1 NC_015388:3272079-3272378 [Desulfobacca acetoxidans DSM 11109]
MLMHNPPHPGEILKQLCLEPLGLTITEAAQALGISRKSLSAIVNGRAGISPEMAIRLSLAFDTTAASWLNQQVMFDLWKA
EQHRGNLKVKSLVTVQLAE

Nucleotide

• Length: 300 bp

>AT25190 NC_015388:3272079-3272378 [Desulfobacca acetoxidans DSM 11109]
ATGCTTATGCACAACCCACCCCATCCTGGAGAAATTCTCAAGCAGCTTTGCCTGGAACCCTTGGGCTTAACCATCACCGA
GGCTGCTCAAGCTTTGGGGATAAGCCGGAAATCATTATCTGCCATTGTCAATGGCCGGGCCGGCATCAGCCCGGAAATGG
CTATCCGGTTATCGTTGGCTTTTGATACCACGGCTGCAAGCTGGCTGAACCAGCAGGTCATGTTCGATCTTTGGAAGGCA
GAGCAACACCGAGGCAATCTCAAAGTTAAAAGCCTGGTAACAGTTCAGCTAGCTGAGTAA

Structures

Toxin

• AlphaFold DB: F2NIZ2