Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1107946..1108673 Replicon chromosome
Accession NZ_CP101310
Organism Escherichia coli strain 99-3311

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag NMK62_RS05460 Protein ID WP_000547563.1
Coordinates 1107946..1108257 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NMK62_RS05465 Protein ID WP_000126294.1
Coordinates 1108254..1108673 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK62_RS05430 (1103091) 1103091..1104800 + 1710 WP_001288147.1 formate hydrogenlyase subunit HycE -
NMK62_RS05435 (1104810) 1104810..1105352 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NMK62_RS05440 (1105352) 1105352..1106119 + 768 WP_000067400.1 formate hydrogenlyase subunit HycG -
NMK62_RS05445 (1106116) 1106116..1106526 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NMK62_RS05450 (1106519) 1106519..1106986 + 468 WP_000132960.1 hydrogenase maturation peptidase HycI -
NMK62_RS05455 (1107029) 1107029..1107784 + 756 WP_001400787.1 hypothetical protein -
NMK62_RS05460 (1107946) 1107946..1108257 + 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
NMK62_RS05465 (1108254) 1108254..1108673 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NMK62_RS05470 (1108787) 1108787..1110211 - 1425 WP_000110310.1 6-phospho-beta-glucosidase AscB -
NMK62_RS05475 (1110220) 1110220..1111677 - 1458 WP_001107816.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NMK62_RS05480 (1111938) 1111938..1112948 + 1011 WP_032161993.1 DNA-binding transcriptional regulator AscG -
NMK62_RS05485 (1113097) 1113097..1113624 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T251886 WP_000547563.1 NZ_CP101310:1107946-1108257 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT251886 WP_000126294.1 NZ_CP101310:1108254-1108673 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References