Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 685376..686103 Replicon chromosome
Accession NZ_CP101310
Organism Escherichia coli strain 99-3311

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NMK62_RS03460 Protein ID WP_000550189.1
Coordinates 685376..685690 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NMK62_RS03465 Protein ID WP_000560266.1
Coordinates 685687..686103 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK62_RS03440 (681533) 681533..682519 - 987 WP_024221603.1 Gfo/Idh/MocA family oxidoreductase -
NMK62_RS03445 (682598) 682598..683290 - 693 WP_000942541.1 vancomycin high temperature exclusion protein -
NMK62_RS03450 (683367) 683367..683870 - 504 WP_001295542.1 M48 family metallopeptidase -
NMK62_RS03455 (683955) 683955..685091 + 1137 WP_000018698.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NMK62_RS03460 (685376) 685376..685690 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NMK62_RS03465 (685687) 685687..686103 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NMK62_RS03470 (686148) 686148..688166 - 2019 WP_000121443.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NMK62_RS03475 (688470) 688470..690014 - 1545 WP_000258466.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T251882 WP_000550189.1 NZ_CP101310:685376-685690 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT251882 WP_000560266.1 NZ_CP101310:685687-686103 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References