Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5297819..5298421 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NMK59_RS26655 Protein ID WP_000897305.1
Coordinates 5298110..5298421 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NMK59_RS26650 Protein ID WP_000356397.1
Coordinates 5297819..5298109 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS26625 (5292894) 5292894..5294501 - 1608 WP_001222883.1 dipeptide ABC transporter substrate-binding protein DppA -
NMK59_RS26630 (5294853) 5294853..5295353 - 501 WP_000469069.1 hypothetical protein -
NMK59_RS26635 (5295345) 5295345..5296325 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
NMK59_RS26640 (5296554) 5296554..5296796 - 243 WP_001086388.1 protein YiiF -
NMK59_RS26645 (5297015) 5297015..5297233 - 219 WP_001295676.1 CopG family transcriptional regulator -
NMK59_RS26650 (5297819) 5297819..5298109 - 291 WP_000356397.1 NadS family protein Antitoxin
NMK59_RS26655 (5298110) 5298110..5298421 - 312 WP_000897305.1 hypothetical protein Toxin
NMK59_RS26660 (5298650) 5298650..5299558 + 909 WP_001162704.1 alpha/beta hydrolase -
NMK59_RS26665 (5299622) 5299622..5300563 - 942 WP_001300148.1 fatty acid biosynthesis protein FabY -
NMK59_RS26670 (5300608) 5300608..5301045 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NMK59_RS26675 (5301042) 5301042..5301914 - 873 WP_000920762.1 virulence factor BrkB family protein -
NMK59_RS26680 (5301908) 5301908..5302507 - 600 WP_001295269.1 glucose-1-phosphatase -
NMK59_RS26685 (5302606) 5302606..5303391 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5295345..5296325 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T251853 WP_000897305.1 NZ_CP101302:c5298421-5298110 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT251853 WP_000356397.1 NZ_CP101302:c5298109-5297819 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References