Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4876250..4877049 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag NMK59_RS24560 Protein ID WP_000347267.1
Coordinates 4876585..4877049 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NMK59_RS24555 Protein ID WP_001307405.1
Coordinates 4876250..4876585 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS24540 (4872035) 4872035..4872805 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NMK59_RS24545 (4872821) 4872821..4874155 - 1335 WP_000599627.1 galactarate/glucarate/glycerate transporter GarP -
NMK59_RS24550 (4874530) 4874530..4876101 + 1572 WP_001273741.1 galactarate dehydratase -
NMK59_RS24555 (4876250) 4876250..4876585 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NMK59_RS24560 (4876585) 4876585..4877049 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NMK59_RS24565 (4877104) 4877104..4877913 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NMK59_RS24570 (4878162) 4878162..4879442 + 1281 WP_000681952.1 tagatose-bisphosphate aldolase subunit KbaZ -
NMK59_RS24575 (4879465) 4879465..4879938 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NMK59_RS24580 (4879949) 4879949..4880728 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NMK59_RS24585 (4880718) 4880718..4881596 + 879 WP_001399813.1 PTS N-acetylgalactosamine transporter subunit IID -
NMK59_RS24590 (4881614) 4881614..4882048 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4864202..4877049 12847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T251851 WP_000347267.1 NZ_CP101302:4876585-4877049 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT251851 WP_001307405.1 NZ_CP101302:4876250-4876585 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References