Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1136483..1137024 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag NMK59_RS05350 Protein ID WP_000615976.1
Coordinates 1136483..1136761 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag NMK59_RS05355 Protein ID WP_000729704.1
Coordinates 1136764..1137024 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS05335 (1134065) 1134065..1134643 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
NMK59_RS05340 (1134849) 1134849..1135616 + 768 WP_000333380.1 class II glutamine amidotransferase -
NMK59_RS05345 (1135587) 1135587..1136327 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NMK59_RS05350 (1136483) 1136483..1136761 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NMK59_RS05355 (1136764) 1136764..1137024 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NMK59_RS05360 (1137210) 1137210..1137983 + 774 WP_000543899.1 C40 family peptidase -
NMK59_RS05365 (1138040) 1138040..1138396 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
NMK59_RS05370 (1138389) 1138389..1138667 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
NMK59_RS05375 (1138772) 1138772..1140484 - 1713 Protein_1044 flagellar biosynthesis protein FlhA -
NMK59_RS05380 (1140456) 1140456..1141241 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T251828 WP_000615976.1 NZ_CP101302:c1136761-1136483 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT251828 WP_000729704.1 NZ_CP101302:c1137024-1136764 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References