251822

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5635095..5635697 Replicon chromosome
Accession NZ_CP101292
Organism Escherichia coli strain 2003-3014

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NMK58_RS28945 Protein ID WP_000897305.1
Coordinates 5635386..5635697 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NMK58_RS28940 Protein ID WP_000356397.1
Coordinates 5635095..5635385 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK58_RS28915 (5630641) 5630641..5631276 + 636 WP_000829008.1 formate dehydrogenase cytochrome b556 subunit -
NMK58_RS28920 (5631273) 5631273..5632202 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
NMK58_RS28925 (5632602) 5632602..5633582 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
NMK58_RS28930 (5633811) 5633811..5634053 - 243 WP_001086390.1 protein YiiF -
NMK58_RS28935 (5634272) 5634272..5634490 - 219 WP_001295676.1 CopG family transcriptional regulator -
NMK58_RS28940 (5635095) 5635095..5635385 - 291 WP_000356397.1 NadS family protein Antitoxin
NMK58_RS28945 (5635386) 5635386..5635697 - 312 WP_000897305.1 hypothetical protein Toxin
NMK58_RS28950 (5635926) 5635926..5636834 + 909 WP_001162704.1 alpha/beta hydrolase -
NMK58_RS28955 (5636898) 5636898..5637839 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NMK58_RS28960 (5637884) 5637884..5638321 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NMK58_RS28965 (5638318) 5638318..5639190 - 873 WP_000920762.1 virulence factor BrkB family protein -
NMK58_RS28970 (5639184) 5639184..5639783 - 600 WP_001315111.1 glucose-1-phosphatase -
NMK58_RS28975 (5639882) 5639882..5640667 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5632602..5633582 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T251822 WP_000897305.1 NZ_CP101292:c5635697-5635386 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT251822 WP_000356397.1 NZ_CP101292:c5635385-5635095 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References