Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5140058..5140857 Replicon chromosome
Accession NZ_CP101292
Organism Escherichia coli strain 2003-3014

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NMK58_RS26535 Protein ID WP_000347273.1
Coordinates 5140393..5140857 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NMK58_RS26530 Protein ID WP_001307405.1
Coordinates 5140058..5140393 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK58_RS26515 (5135843) 5135843..5136613 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NMK58_RS26520 (5136629) 5136629..5137963 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NMK58_RS26525 (5138338) 5138338..5139909 + 1572 WP_001273779.1 galactarate dehydratase -
NMK58_RS26530 (5140058) 5140058..5140393 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NMK58_RS26535 (5140393) 5140393..5140857 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NMK58_RS26540 (5140912) 5140912..5141721 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
NMK58_RS26545 (5141970) 5141970..5143250 + 1281 WP_000681914.1 tagatose-bisphosphate aldolase subunit KbaZ -
NMK58_RS26550 (5143273) 5143273..5143746 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NMK58_RS26555 (5143757) 5143757..5144536 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NMK58_RS26560 (5144526) 5144526..5145404 + 879 WP_001341890.1 PTS N-acetylgalactosamine transporter subunit IID -
NMK58_RS26565 (5145422) 5145422..5145856 + 435 WP_000948822.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5129402..5140857 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T251821 WP_000347273.1 NZ_CP101292:5140393-5140857 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT251821 WP_001307405.1 NZ_CP101292:5140058-5140393 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References