Detailed information of TA system TA025181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2912481..2913210
• Replicon: chromosome
• Accession: NC_015381
• Organism: Burkholderia gladioli BSR3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F2LFJ4
• Locus tag: BGLA_RS12575
• Protein ID: WP_013698487.1
• Coordinates: 2912899..2913210 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: F2LFJ3
• Locus tag: BGLA_RS12570
• Protein ID: WP_013698486.1
• Coordinates: 2912481..2912897 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGLA_RS39990	2908026..2908583	+	558	WP_013698480.1	toll/interleukin-1 receptor domain-containing protein	-
BGLA_RS12550	2908725..2909387	-	663	WP_013698481.1	uracil-DNA glycosylase	-
BGLA_RS12555	2909384..2910523	-	1140	WP_013698482.1	nucleoside triphosphate pyrophosphohydrolase family protein	-
BGLA_RS39995	2910527..2911153	-	627	WP_013698483.1	hypothetical protein	-
BGLA_RS12560	2911153..2911620	-	468	WP_013698484.1	TIR domain-containing protein	-
BGLA_RS12565	2911933..2912316	+	384	WP_013698485.1	AlpA family phage regulatory protein	-
BGLA_RS12570	2912481..2912897	-	417	WP_013698486.1	transcriptional regulator	Antitoxin
BGLA_RS12575	2912899..2913210	-	312	WP_013698487.1	type II toxin-antitoxin system HigB family toxin	Toxin
BGLA_RS12580	2913291..2914547	-	1257	WP_013698488.1	site-specific integrase	-
BGLA_RS12585	2914859..2916478	-	1620	WP_013698489.1	glutamine-hydrolyzing GMP synthase	-
BGLA_RS41215	2916861..2917280	-	420	Protein_2543	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2871875..2914547	42672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12258.94 Da
• Isoelectric Point: 9.6242

>T25181 WP_013698487.1 NC_015381:c2913210-2912899 [Burkholderia gladioli BSR3]
MHVISRKPFSDAAKAYPNDAEAIDRTYRVLRSGDFDTPQALRQVFPSLDNFKYKDKWWVLDIGGNNLRLIAFIEFRDNRM
YVKHITTHAEYDKLCKRYRKEGD

Nucleotide

• Length: 312 bp

>T25181 NC_015381:c2913210-2912899 [Burkholderia gladioli BSR3]
ATGCACGTCATATCCAGAAAGCCGTTCAGCGACGCAGCAAAGGCTTATCCGAACGACGCCGAGGCGATCGACCGCACCTA
CCGGGTGCTGCGATCCGGCGATTTCGATACACCGCAAGCACTGCGACAGGTTTTTCCGTCGCTGGATAACTTCAAGTACA
AGGACAAGTGGTGGGTGCTGGACATCGGTGGCAACAATCTTCGACTGATTGCCTTCATCGAGTTTCGCGACAACCGCATG
TACGTCAAGCACATCACAACCCATGCCGAGTACGACAAGCTTTGCAAACGCTACCGGAAGGAGGGCGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15184.26 Da
• Isoelectric Point: 4.6805

>AT25181 WP_013698486.1 NC_015381:c2912897-2912481 [Burkholderia gladioli BSR3]
MGFAAIKERAHALFDEAGFIGHIANEDDYAQALALMDELVEDYEANRPLIEILARSIEIWENESDEFAAFNARVAKLGGV
DVLRLLMEQHGLGVADLPEIGSKSLVSKILNGRGRNLTRDHIAALSKRFSVSPAVFFS

Nucleotide

• Length: 417 bp

>AT25181 NC_015381:c2912897-2912481 [Burkholderia gladioli BSR3]
ATGGGATTTGCCGCCATCAAGGAACGAGCCCATGCCTTGTTCGACGAGGCGGGTTTCATTGGCCATATCGCCAATGAAGA
TGATTACGCCCAGGCGCTGGCGTTGATGGATGAACTGGTCGAAGACTACGAGGCCAACCGCCCACTGATCGAGATCCTGG
CCCGTTCCATCGAAATATGGGAAAACGAGTCCGACGAGTTTGCGGCGTTCAACGCGCGCGTGGCGAAGCTGGGCGGGGTC
GATGTGCTGCGATTGTTGATGGAGCAGCATGGACTGGGCGTGGCAGATCTGCCGGAGATCGGTAGTAAGTCACTGGTATC
CAAGATTCTCAACGGTCGGGGACGCAACTTGACCCGCGACCACATCGCAGCATTGAGCAAGCGGTTCTCAGTCAGTCCGG
CTGTTTTCTTCAGCTAA

Structures

Toxin

• AlphaFold DB: F2LFJ4

Antitoxin

• AlphaFold DB: F2LFJ3