Detailed information of TA system TA025180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5670727..5671382
• Replicon: chromosome
• Accession: NC_015379
• Organism: Pseudomonas brassicacearum subsp. brassicacearum NFM421

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: PSEBR_RS24935
• Protein ID: WP_013694185.1
• Coordinates: 5671038..5671382 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PSEBR_RS24930
• Protein ID: WP_003205391.1
• Coordinates: 5670727..5671041 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEBR_RS24910	5666773..5667690	+	918	WP_013694183.1	LysR family transcriptional regulator	-
PSEBR_RS24915	5667840..5668853	+	1014	WP_003205388.1	diguanylate cyclase	-
PSEBR_RS24920	5668945..5669688	+	744	WP_013694184.1	DUF72 domain-containing protein	-
PSEBR_RS24925	5669810..5670514	+	705	WP_003205390.1	hypothetical protein	-
PSEBR_RS24930	5670727..5671041	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
PSEBR_RS24935	5671038..5671382	-	345	WP_013694185.1	hypothetical protein	Toxin
PSEBR_RS24940	5671522..5672409	-	888	WP_003205393.1	heme o synthase	-
PSEBR_RS24945	5672421..5672756	-	336	WP_003205394.1	cytochrome o ubiquinol oxidase subunit IV	-
PSEBR_RS24950	5672757..5673383	-	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
PSEBR_RS24955	5673387..5675417	-	2031	WP_003205396.1	cytochrome o ubiquinol oxidase subunit I	-
PSEBR_RS24960	5675421..5676368	-	948	WP_003205397.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T25180 WP_013694185.1 NC_015379:c5671382-5671038 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T25180 NC_015379:c5671382-5671038 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGCGTACCCTATTTTTTGAAACGACTACATTCACCGACACGGTTGGCCATTACCTGACTGATGATGAGTATCGCCTGCT
TCAGTCCTATATGCTGCAGCATCCAGAGGTTGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAACGTCGTGGTAAAGGGAAGCGAGGCGGGTTGCGCGTTATTTACTATTGGCTCATGAACGATCGTCAGTTCTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGAAAACCTGACCTCCGAGCAAGAGAAGACGCTCAAACGCGCCATAGAAGCTGA
GTTGAAAGTACGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT25180 WP_003205391.1 NC_015379:c5671041-5670727 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT25180 NC_015379:c5671041-5670727 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGAAAAAGCGCGATCTGTTTGCCGAGATGATGCAAGGCGTCGAGGAGATGGCCGCCCACCGGGAGGGCAAGATTACCCT
TCGCCAGATATCGATCGAAGACAAACCGGTTCCCGAGGTGTCTGCCCAGGAAATCGTGGCGTTGCGCGACAAGCTCCACA
TGTCTCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCAGGTACGCTCAGGAACTGGGAGCAGGAGAAGTCCAAGCCT
AACGCCCAGGCGGCTTTGTTGATCAAGCTGGTCGAGAAGTTTCCAGATATGGTCGAACGGCTTGGCGCTGTTTGA

Structures

Toxin

• AlphaFold DB: G8QAX9