Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1058356..1058891 Replicon chromosome
Accession NZ_CP101292
Organism Escherichia coli strain 2003-3014

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag NMK58_RS05020 Protein ID WP_032183258.1
Coordinates 1058356..1058643 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag NMK58_RS05025 Protein ID WP_000729704.1
Coordinates 1058631..1058891 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK58_RS05005 (1055938) 1055938..1056516 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
NMK58_RS05010 (1056722) 1056722..1057489 + 768 WP_000333380.1 class II glutamine amidotransferase -
NMK58_RS05015 (1057460) 1057460..1058200 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NMK58_RS05020 (1058356) 1058356..1058643 - 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NMK58_RS05025 (1058631) 1058631..1058891 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NMK58_RS05030 (1059077) 1059077..1059850 + 774 WP_000543897.1 C40 family peptidase -
NMK58_RS05035 (1059907) 1059907..1060263 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
NMK58_RS05040 (1060256) 1060256..1060534 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
NMK58_RS05045 (1060639) 1060639..1062732 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
NMK58_RS05050 (1062716) 1062716..1063855 - 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T251799 WP_032183258.1 NZ_CP101292:c1058643-1058356 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT251799 WP_000729704.1 NZ_CP101292:c1058891-1058631 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References