Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4044496..4045295 Replicon chromosome
Accession NZ_CP101274
Organism Escherichia coli strain EC21Z-014

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag NML17_RS19710 Protein ID WP_000347266.1
Coordinates 4044831..4045295 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NML17_RS19705 Protein ID WP_001307405.1
Coordinates 4044496..4044831 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML17_RS19690 (4040281) 4040281..4041051 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NML17_RS19695 (4041067) 4041067..4042401 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NML17_RS19700 (4042776) 4042776..4044347 + 1572 WP_001273741.1 galactarate dehydratase -
NML17_RS19705 (4044496) 4044496..4044831 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NML17_RS19710 (4044831) 4044831..4045295 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NML17_RS19715 (4045350) 4045350..4046159 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NML17_RS19720 (4046408) 4046408..4047688 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
NML17_RS19725 (4047711) 4047711..4048184 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NML17_RS19730 (4048195) 4048195..4048974 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NML17_RS19735 (4048964) 4048964..4049842 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
NML17_RS19740 (4049860) 4049860..4050294 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4033508..4045295 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T251774 WP_000347266.1 NZ_CP101274:4044831-4045295 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT251774 WP_001307405.1 NZ_CP101274:4044496-4044831 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References