Detailed information of TA system TA251751

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93012..93433
• Replicon: plasmid pEC21Z048-116K
• Accession: NZ_CP101270
• Organism: Escherichia coli strain EC21Z-048

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: NML18_RS23910
• Protein ID: WP_096937776.1
• Coordinates: 93012..93137 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	93235..93433 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NML18_RS23875 (88409)	88409..89098	-	690	WP_254837473.1	conjugal transfer transcriptional regulator TraJ	-
NML18_RS23880 (89285)	89285..89668	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
NML18_RS23885 (90001)	90001..90591	+	591	WP_175345273.1	transglycosylase SLT domain-containing protein	-
NML18_RS23890 (90887)	90887..91708	-	822	WP_001234417.1	DUF932 domain-containing protein	-
NML18_RS23895 (91827)	91827..92114	-	288	WP_000107542.1	hypothetical protein	-
NML18_RS23900 (92139)	92139..92345	-	207	WP_000547965.1	hypothetical protein	-
NML18_RS23905 (92415)	92415..92711	+	297	Protein_113	hypothetical protein	-
NML18_RS23910 (93012)	93012..93137	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
NML18_RS23915 (93079)	93079..93228	-	150	Protein_115	DUF5431 family protein	-
- (93235)	93235..93433	-	199	NuclAT_0	-	Antitoxin
- (93235)	93235..93433	-	199	NuclAT_0	-	Antitoxin
- (93235)	93235..93433	-	199	NuclAT_0	-	Antitoxin
- (93235)	93235..93433	-	199	NuclAT_0	-	Antitoxin
NML18_RS23920 (93402)	93402..94164	-	763	Protein_116	plasmid SOS inhibition protein A	-
NML18_RS23925 (94161)	94161..94595	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
NML18_RS23930 (94650)	94650..96608	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
NML18_RS23935 (96674)	96674..96907	-	234	WP_000005990.1	DUF905 family protein	-
NML18_RS23940 (96964)	96964..97503	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
NML18_RS23945 (97821)	97821..98330	+	510	WP_071594366.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeI / faeH / faeF / faeE / faeD / faeC	1..115682	115682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T251751 WP_096937776.1 NZ_CP101270:c93137-93012 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 199 bp

>AT251751 NZ_CP101270:c93433-93235 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin