Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3743108..3743649 Replicon chromosome
Accession NZ_CP101269
Organism Escherichia coli strain EC21Z-048

Toxin (Protein)


Gene name yafQ Uniprot ID A7ZHY5
Locus tag NML18_RS18300 Protein ID WP_012000916.1
Coordinates 3743371..3743649 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag NML18_RS18295 Protein ID WP_000729704.1
Coordinates 3743108..3743368 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML18_RS18270 (3738891) 3738891..3739676 - 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -
NML18_RS18275 (3739648) 3739648..3741360 + 1713 Protein_3578 flagellar biosynthesis protein FlhA -
NML18_RS18280 (3741465) 3741465..3741743 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
NML18_RS18285 (3741736) 3741736..3742092 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
NML18_RS18290 (3742149) 3742149..3742922 - 774 WP_000543899.1 C40 family peptidase -
NML18_RS18295 (3743108) 3743108..3743368 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NML18_RS18300 (3743371) 3743371..3743649 + 279 WP_012000916.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NML18_RS18305 (3743805) 3743805..3744545 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NML18_RS18310 (3744516) 3744516..3745283 - 768 WP_000333380.1 class II glutamine amidotransferase -
NML18_RS18315 (3745489) 3745489..3746067 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T251742 WP_012000916.1 NZ_CP101269:3743371-3743649 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT251742 WP_000729704.1 NZ_CP101269:3743108-3743368 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZHY5

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References