Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 594923..595722 Replicon chromosome
Accession NZ_CP101269
Organism Escherichia coli strain EC21Z-048

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag NML18_RS02915 Protein ID WP_000347267.1
Coordinates 594923..595387 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NML18_RS02920 Protein ID WP_001307405.1
Coordinates 595387..595722 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML18_RS02890 (590376) 590376..591254 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NML18_RS02895 (591244) 591244..592023 - 780 WP_254837356.1 PTS N-acetylgalactosamine transporter subunit IIC -
NML18_RS02900 (592034) 592034..592507 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NML18_RS02905 (592530) 592530..593810 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
NML18_RS02910 (594059) 594059..594868 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NML18_RS02915 (594923) 594923..595387 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NML18_RS02920 (595387) 595387..595722 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NML18_RS02925 (595871) 595871..597442 - 1572 WP_001273741.1 galactarate dehydratase -
NML18_RS02930 (597817) 597817..599151 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NML18_RS02935 (599167) 599167..599937 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 588586..607657 19071
- flank IS/Tn - - 589033..590241 1208
- inside Genomic island - - 580297..607657 27360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T251730 WP_000347267.1 NZ_CP101269:c595387-594923 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT251730 WP_001307405.1 NZ_CP101269:c595722-595387 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References