Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629289..630088 Replicon chromosome
Accession NZ_CP101251
Organism Escherichia coli strain EC21Z-083

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag NML21_RS03050 Protein ID WP_000347251.1
Coordinates 629289..629753 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NML21_RS03055 Protein ID WP_001307405.1
Coordinates 629753..630088 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML21_RS03020 (624290) 624290..624724 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NML21_RS03025 (624742) 624742..625620 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NML21_RS03030 (625610) 625610..626389 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NML21_RS03035 (626400) 626400..626873 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NML21_RS03040 (626896) 626896..628176 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
NML21_RS03045 (628425) 628425..629234 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NML21_RS03050 (629289) 629289..629753 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NML21_RS03055 (629753) 629753..630088 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NML21_RS03060 (630237) 630237..631808 - 1572 WP_001273761.1 galactarate dehydratase -
NML21_RS03065 (632183) 632183..633517 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
NML21_RS03070 (633533) 633533..634303 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T251651 WP_000347251.1 NZ_CP101251:c629753-629289 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT251651 WP_001307405.1 NZ_CP101251:c630088-629753 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References