Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1086342..1087069 Replicon chromosome
Accession NZ_CP101240
Organism Escherichia coli strain EC21Z-101

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag NML23_RS05280 Protein ID WP_000547564.1
Coordinates 1086342..1086653 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NML23_RS05285 Protein ID WP_000126294.1
Coordinates 1086650..1087069 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML23_RS05250 (1081484) 1081484..1083193 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
NML23_RS05255 (1083203) 1083203..1083745 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
NML23_RS05260 (1083745) 1083745..1084512 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NML23_RS05265 (1084509) 1084509..1084919 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NML23_RS05270 (1084912) 1084912..1085382 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NML23_RS05275 (1085407) 1085407..1086168 + 762 WP_001026446.1 hypothetical protein -
NML23_RS05280 (1086342) 1086342..1086653 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
NML23_RS05285 (1086650) 1086650..1087069 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NML23_RS05290 (1087183) 1087183..1088607 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
NML23_RS05295 (1088616) 1088616..1090073 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NML23_RS05300 (1090333) 1090333..1091343 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
NML23_RS05305 (1091492) 1091492..1092019 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T251604 WP_000547564.1 NZ_CP101240:1086342-1086653 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT251604 WP_000126294.1 NZ_CP101240:1086650-1087069 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References