Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3539250..3539944 Replicon chromosome
Accession NZ_CP101233
Organism Escherichia coli strain EC21Z-137

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag NML24_RS17315 Protein ID WP_001263493.1
Coordinates 3539250..3539648 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag NML24_RS17320 Protein ID WP_000554757.1
Coordinates 3539651..3539944 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3534910) 3534910..3534990 - 81 NuclAT_9 - -
- (3534910) 3534910..3534990 - 81 NuclAT_9 - -
- (3534910) 3534910..3534990 - 81 NuclAT_9 - -
- (3534910) 3534910..3534990 - 81 NuclAT_9 - -
NML24_RS17285 (3534250) 3534250..3535494 - 1245 WP_000189541.1 esterase FrsA -
NML24_RS17290 (3535586) 3535586..3536044 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NML24_RS17295 (3536305) 3536305..3537762 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
NML24_RS17300 (3537819) 3537819..3538340 - 522 Protein_3379 peptide chain release factor H -
NML24_RS17305 (3538339) 3538339..3538542 - 204 Protein_3380 RtcB family protein -
NML24_RS17310 (3538788) 3538788..3539240 - 453 WP_180759961.1 GNAT family N-acetyltransferase -
NML24_RS17315 (3539250) 3539250..3539648 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NML24_RS17320 (3539651) 3539651..3539944 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NML24_RS17325 (3539996) 3539996..3541051 - 1056 WP_001226164.1 DNA polymerase IV -
NML24_RS17330 (3541122) 3541122..3541907 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
NML24_RS17335 (3541879) 3541879..3543591 + 1713 Protein_3386 flagellar biosynthesis protein FlhA -
NML24_RS17340 (3543815) 3543815..3544312 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T251589 WP_001263493.1 NZ_CP101233:c3539648-3539250 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT251589 WP_000554757.1 NZ_CP101233:c3539944-3539651 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References